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Antimicrobial Activity of the Rhizomes of Curcuma Zedoaria

Mohammad Shahriar
Department of Pharmacy, The University of Asia Pacific, House No. 73, Road No. 5A, Dhanmondi, Dhaka-1209, Bangladesh.

Abstract/ Executive Summary:

Successive extraction of the dried rhizomes of Curcuma zedoaria Rocs. provided n-hexane, ethyl acetate and methanol extracts. The antimicrobial activity of the different extracts of Curcuma zedoaria was investigated at an initial dose of 500 μg/disc against gram positive, gram negative bacteria and fungi. The methanol extract of the herb showed significant activity against some tested gram negative bacteria. The ethyl acetate extract exhibited moderate activity while the n-hexane showed little activity against some gram negative bacteria.

Keywords: Curcuma zedoaria, Rhizomes, Antimicrobial activity

Location: National Herbarium of Mirpur, Dhaka.

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To investigate the antimicrobial activity of the rhizomes of Curcuma zedoaria

Methodology:

The rhizomes of Curcuma zedoaria Rosc. were collected from Sylhet and identified properly by a taxonomist of the National Herbarium of Mirpur, Dhaka. The rhizomes were washed and cut into small pieces then dried and pulverized into a coarse powder. About 800 gm of dried powder of the rhizome was percolated successively with n-hexane, ethyl acetate and methanol (3 liters of each) to obtain n-hexane extract (1.8 gm), ethyl acetate extract (2.5 gm) and methanol extract (4.5 gm). All the extracts were dissolved in methanol separately, kept in refrigerator for 24 hours at 4 ºC for precipitation of fatty materials, filtered off through a piece of folded cloth and finally concentrated to get defatted extracted. About 1.5 gm of the n-hexane extracted was subjected to silica gel column chromatography with n-hexane/ethyl acetate gradient, and four fraction namely, HF-1 (n-hexane-ethyl acetate; 9 : 1), HF-2 (n-hexane-ethyl acetate; 7 : 3), HF-3 (n-hexane-ethyl acetate; 5 : 5), HF-4 (n-hexane-ethyl acetate; 1 : 9) were obtained. The column fraction HF-2 gave crystal on evaporation of the solvent. The crystals were separated and washed with n-hexane twice. Then it was dissolved in ethyl acetate, filtered off and allowed to recrystallize. The pure strains of both Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, B. cereus, B. megaterium, Sarcina lutea), Gram negative bacteria (Salmonella paratyphi A, S. paratyphi B, Escherichia coli, Shigella boydii, Sh. dysenteriae, Sh. sonei, Vibro cholerae, V. mimicus, Klebsiella sp., Pseudomonas aeruginosa) and four fungi (Saccharromyces cerevaceae, Aspergillus niger, Candida albicans, Rhizopus oryzae ) were collected from Department of Microbiology, University of Dhaka.

Source of Information: Journal of Bangladesh Academy of Sciences, Vol. 34, No. 2, 201-203, 2010, ISSN 0378-8121

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/jbas.v34i2.6867

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The methanol extract of the herb showed significant activity against some tested gram negative bacteria. The ethyl acetate extract exhibited moderate activity while the n-hexane showed little activity against some gram negative bacteria. No extract was found to be active against gram positive bacteria. On the other hand, in the antifungal activity testing none of the extracts showed any activity against the tested organisms.

Knowledge Management: Journal