Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Standardization of Protocol for in Vitro Regeneration of Mint (mintha Sp.)

M. A. Hoque
Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur-1706, Bangladesh.

K. T. Akter
Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur-1706, Bangladesh.

Abstract/ Executive Summary:

An attempt was made to standardize the protocol for in vitro regeneration of mint at the Tissue Culture Laboratory of the Bangabandhu Sheikh Mujibur Rahman Agricultural University during December 2012 to June 2013. Four levels of media (viz., M₁= Full strength MS media without growth regulators, M₂= Half strength MS media without growth regulators, M₃= Full strength MS media supplemented with growth regulators, and M₄= Half strength MS media supplemented with growth regulators) along with three types of explant (viz., E₁= Shoot tip, E₂= Node, and E₃= Petiole with a portion of leaf) were used in the experiment. Highest success (100%) in shoot regeneration was recorded in M₁E₁, M₁E₂, M3E₁, M₃E₂, M₄E₁, and M₄E₂ while, the treatment combination M₂E₃, and M₄E₃ produced no shoot in vitro. On the other hand, highest success (100%) in root generation was recorded in M₄E₁, M₃E₁, and M₁E₂. Shoot tip and nodal segment along with full strength MS media supplemented with 1.0 mg/L of NAA and BAP were found to perform best in number of shoots, length of longest root (cm), number of visible internodes, number of roots and length of longest shoot (cm) formation. On overall consideration, shoot tip and nodal segment along with full strength MS media supplemented with growth regulators (1mg/L NAA and 1mg/L BAP) may be used in the case of in vitro regeneration of mint.

Keywords: MS medium, Explants, Mintha sp.

Location: Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur

Start Date: 00-12-2012

End Date: 00-06-2013

Program Area: Development of Host and Medicinal Plants

Commodity: Mint

Objectives:

The present experiment had been designed to develop protocol for in vitro propagation of mint.

Methodology:

The experiment was conducted at the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur in December 2012 to June 2013. Four levels of media (viz., M₁= Full strength MS media without growth regulators, M₂= Half strength MS media without growth regulators, M₃= Full strength MS media supplemented with NAA @ 1.0 mg/L and BAP @ 1.0 mg/L and M₄= Half strength MS media supplemented with NAA @ 1.0 mg/L and BAP @ 1.0 mg/L) along with 3 types of ex-plant (viz. E₁= Shoot tip, E₂= Node, and E₃= Petiole with a portion of leaf) were used in the experiment. There were 12 treatment combinations in the experiment and the experiment was set on 1 December 2012 following Completely Randomized Design (CRD) with 10 replications. Shoot tips, nodes, and petioles excised from previously grown healthy mint plants were washed under running tap water followed by soaking in 2-3 drops of Tween-20 for 5 minutes. Therefore, the plant parts were rinsed in distilled water for 2-3 times. It was followed by surface sterilization using mercuric chloride (0.1% w/v) in laminar flow cabinet and finally rinsed with 3-4 times in a sterile double distilled water to remove traces of mercuric chloride. The first and second nodes and shoot meristems (2-3 mm) were excised from the sterilized plant parts, while petioles with a small portion of leaves were excised in 3-4 mm pieces. The ex-plants were inoculated by inserting their cut ends in the medium. The medium were prepared by mixing required amount of MS powder supplemented with or without growth regulators and 3% (w/v) sucrose. The mixture was then stirred in an electric stir for dissolving sucrose and MS powder. Then the pH of the medium was adjusted to 5.7 and agar powder was added @ 0.8% (w/v) as solidifying agent. Therefore, the medium was heated into a microwave oven for 3-5 minutes for dissolving the ingredients completely and was autoclaved at 121⁰C, 15 psi for 20 minutes. The cultures were kept in growth chambers at 20±2⁰C under 16h light photoperiod using Philips day light florescent lamps under light intensity of 10000 lux. Scoring was done after six weeks of culture by counting all shoots and roots on the explants. Therefore, the shoots and nodes of each plantlet were excised carefully and sub-cultured for multiplication. After four weeks, well rooted plantlets were removed from the culture and washed with sterile distilled water to remove the media adhering with roots. These were then transferred to plastic pots containing sterilized soil mixture composed of garden soil and cowdung (1:1). After 1 week, the plastic pots were gradually exposed to outside the laboratory (normal condition) for next two weeks and then transferred to the main field. The collected data were analyzed statistically using computer MSTAT-C program and the means were separated by Duncan's Multiple Range Test (DMRT).

Source of Information: Ann. Bangladesh Agric. (2014) 18 (1): 15-25; ISSN 1025-482X.

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

For in vitro regeneration of mint, shoot tip or nodal segments as explants and full strength MS media supplemented with growth regulators (1mg/L NAA and 1mg/L BAP) may be used.

Knowledge Management: Journal