N. Krishna Kundu
Department of Pharmacy, Jahangirnagar University, Dhaka
M. Obayed Ullah
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD - 4072, Australia
Kaiser Hamid
Department of Pharmacy, East West University, Dhaka, Bangladesh
Kaniz Fatima Urmi
Department of Pharmacy, Jahangirnagar University, Dhaka
Israt Jahan Bulbul
Department of Pharmacy, Southeast University, Dhaka, Bangladesh
Muhammad Atikul Islam Khan
Department of Microbiology, University of Dhaka, Bangladesh
Momita Akter
Department of Pharmacy, Jahangirnagar University, Dhaka
M.S.K. Choudhuri
Department of Pharmacy, Jahangirnagar University, Dhaka
Sulavajrini vatika, Ayurvedic, Lipid profile, Kidney function, Liver function
Department of Pharmacy, Jahangirnagar University, Dhaka
Development of Host and Medicinal Plants
Performance
Dose and route of administration: To accomplish the study of intended biochemical parameters, SBB was collected from Sree Durga Aushadhalaya Ltd., Chittagong. The liquid was administered at a volume such that it would permit optimal dosage accuracy without contributing much to the total increase in the body fluid. The drugs were administered per oral route at a single dose of 100 mg kg-1 b.wt. day-1.
Experimental animal: For the study, forty eight-week old albino rats (Rattus norvegicus: Sprague-Dawley strain) of both sexes, bred and maintained at the Animal House of the Department of Pharmacy, Jahangirnagar University. These animals were apparently healthy and weighed 450-500 g. The animals were housed in a well ventilated hygienic experimental animal house under constant environmental and adequate nutritional conditions throughout the period of the experiment. All of the rats were kept in plastic cages having dimensions of 30x20x13 cm and soft wood shavings were employed as bedding in the cages. Feeding of animals was done ad libitum, along with drinking water and maintained at natural day night cycle. They were fed with “mouse chow” (prepared according to the formula developed at BCSIR, Dhaka). All experiments on rats were carried out in absolute compliance with the ethical guide for care and use of laboratory animals.
Controls: A group of equal number of rat as the drug treated group was simultaneously used in the experiment. They were administered with distilled water as placebo as par the same volume as the drug treated group for the same number of days and this group served as the control. A total of 40 rats were taken for experiment and prior to the experiment, they were randomly divided into 4 groups of 10 animals/sex. Thus, ten rats were taken for each group for both control and the experimental group. After acclimatization, administration of the Ayurvedic medicinal preparation was done by intra-gastric syringe. Administration of the extract was between the hours of 10 am and 12.00 am.
Blood sample preparation: At the due of the 62-day treatment period, the animals were fasted for 18 -24 h after the last administration, the animals were anaesthetized using i.p. Ketamine (500 mg kg-1 i.p.). Blood samples were collected from post vena cava and transferred into heparinized tubes immediately. Blood was then centrifuged for 10 min using bench top centrifuge (MSE Minor, England) to remove red blood cells and recover plasma. Plasma samples were separated and were collected using dry Pasteur pipette and stored in the refrigerator for analyses. All analyses were completed within 24 h of sample collection.
Determination biochemical parameters: To assess the state of the liver and kidney function, measure the lipid profile, biochemical analysis was carried out on plasma. These studies involved analysis of parameters such as total protein, serum albumin, blood urea nitrogen (BUN), bilirubin (total and direct), creatinine and liver enzymes such as Serum Glutamic Oxaloacetic Transaminase (SGOT), Serum Glutamic Pyruvic Transaminase (SGPT) and Alkaline Phosphatase (ALP).
Total protein content of the samples was assayed by the Biuret method. The method of Evelyn and Malloy was employed to determine the serum bilirubin concentration of the samples. The procedure of Tietz was used to determine serum creatinine concentration while the serum urea concentration was determined by the method of Kaplan. Alkaline phosphatase activities were determined using the method as described by Kind and King. The absorbance of all the tests were determined using spectrophotometer (UV-Visible Spectrophotometer). The obtained data was analyzed using unpaired t test and presented as Mean±Standard Error of the Mean (SEM). Statistical Package for Social Science (SPSS) for windows was applied for the analysis of the data. The p = 0.05 was taken to be level of significance.
Pakistan Journal of Biological Sciences, 15: 666-672; ISSN 1028-8880
Journal