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Respiratory Bacterial Flora from Healthy as Well as Respiratory Symptoms’ Subjects

M. Islam
Department of Microbiology and Hygiene, BAU, Mymensingh - 2202, Bangladesh

S. Hussin
Department Medical Microbiology and Immunology, National University Malaysia, Cheras - 56000, Kuala Lumpur - 56000, Malaysia

M.M. Rahman
Department Medical Microbiology and Immunology, National University Malaysia, Cheras - 56000, Kuala Lumpur - 56000, Malaysia

Abstract/ Executive Summary:

Respiratory system is the primary settlement place of opportunistic organisms and considered as chief carrier of common respiratory pathogens. The aim of the study was to know the opportunistic organisms present in the healthy subjects as well as subjects that were suffering from respiratory symptoms. The organisms were identified as per standard bacteriological protocol and pathogenicity tests of the identified organisms were performed in mouse model. Antibiotic sensitivity of the identified organisms was performed. The bacterial flora present in the throat swab of apparently healthy as well as subjects suffering from respiratory symptoms were: Staphylococcus spp. (39.44%) of which Coagulase positive Staphylococcus (21.13%) and Coagulase negative Staphylococcus (18.31%), Klebsiella spp. (19.72%), Pseudomonas spp. (15.49%), Proteus spp. (4.23%), E. coli (9.86%) and Bacillus spp. (11.27%). Among the isolates Staphylococcus, Klebsiella and Pseudomonas were the predominant species. Percentages of identified bacteria were higher in respiratory symptoms exhibiting individuals (53.52%) than apparently healthy individuals (46.48%). All coagulase positive Staphylococcus, Klebsiella spp. and Pseudomonas spp. isolated from respiratory symptoms’ subjects were found to be pathogenic. The isolated bacteria were resistant to amoxicillin and ampicillin but sensitive to ciprofloxacin and norfloxacin. Isolated Pseudomonas spp. showed multidrugs resistant properties. The study provided information about the pathogenic organisms’ present respiratory systems of apparently healthy as well as subjects suffering from respiratory symptoms. The pathogenic natures of the isolated organisms were determined to make aware of scientists as well as clinicians. Antibiotics sensitivity assays would provide information to the clinicians for the selection of appropriate antibiotics to treat their patients.

Keywords: Opportunistic organisms, Respiratory tract, Apparently healthy, Pathogenity, Antibiotic sensitivity

Location: Department of Microbiology and Hygiene, BAU, Mymensingh

Start Date: 00-01-2009

End Date: 00-12-2009

Program Area: Pest Management

Commodity: Diseases

Objectives:

The present research was undertaken to isolate and identify the bacteria from respiratory tract of apparently healthy and subjects suffering from respiratory symptoms and to study their pathogenicity and antibiotic sensitivity.

Methodology:

This study was conducted in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh during the period of January 2009 to December 2009.

Samples: A total of 60 throat swab samples were aseptically collected in which 30 from apparently healthy adult subjects and 30 from respiratory symptoms’ adult subjects with the help of sterile cotton buds. Immediately after collection, the samples were inoculated into nutrient broth. These were then transferred to the laboratory, Department of Microbiology and Hygiene BAU, Mymensingh-2202, Bangladesh.

Isolation and characterization of bacteria: The collected samples were processed as per the procedure of Cheesbrough. Briefly the samples were then inoculated into Blood Agar (BA) media and incubated at 37°C for 24 h. Characteristic colonies from the plates were isolated and then sub cultured to obtain pure culture. The isolated organisms were identified based on colonial morphology, microscopic study and biochemical tests according to standard laboratory methods of Cheesbrough.

Pathogenicity tests: The isolated Staphylococcus, Klebsiella and Pseudomonas organisms were subjected to pathogenicity test in 2 months old mice by intra-peritoneal inoculation to observe their pathogenicity.

Antibiotic sensitivity tests: Antibiotic sensitivity tests were performed using disc diffusion test of the method described by CLSI.

Source of Information: Pakistan Journal of Biological Sciences, 14: 456-460; ISSN 1028-8880

Article Link (Online Journal): DOI: 10.3923/pjbs.2011.456.460; URL: http://scialert.net/abstract/?doi=pjbs.2011.456.460

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was observed from the present study that Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were predominant of the bacteria isolated from throat swab of subjects. Percentages of identified bacteria were higher in subjects suffering from respiratory symptoms than apparently healthy subjects.

Knowledge Management: Journal