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In Vitro Regeneration Potentiality of Oil Seed Brassica Genotypes with Differential Bap Concentration

Mohammad Mahbubul Alam Khan
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202

Lutful Hassan
Professor
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

Syed Dilnawaz Ahmad
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

Asad Hussain Shah
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

Farhat Batool
Neurochemistry and Biochemical Neuropharmacology Research Laboratory, Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan

Abstract/ Executive Summary:

The experiment was conducted at the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. Petiole of six genotypes of oilseed Brassica viz., Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARISarisha-8 and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA and AgNO₃ for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 with the media supplemented with 2 mgL^-1 BAP, 0.1 mgL^-1 NAA and 2.0 mgL^-1 AgNO₃. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS medium supplemented with 2.5 mgL^-1 BAP, 0.1mgL^-1 NAA and 2.0mgL^-1 AgNO₃. Root formation from the regenerants was found best in half MS medium supplemented with 0.5mgL^-1NAA in genotype Tori-7. Regenerated plantlets of 4 genotypes (Tori-7, BARISarisha-8, Kallyania, BARISarisha-7) were successfully established in the field.

Keywords: In vitro, Regeneration, Oil seed, BAP concentration

Location: Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

Start Date: 00-10-2007

End Date: 00-01-2008

Program Area: Variety and Species

Commodity: Mustard

Objectives:

To know the regeneration potentiality of oil seed Brassica genotypes using differential BAP concentration.

Methodology:

The experiment was conducted at the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. Six oilseed Brassica genotypes viz., Tori-7, Sampad, Kallyania of Brassica rapa and BARISarisha-7, BARISarisha-8 and MM 20-3 of Brassica napus were used for this experiment. Seed of Sampad was collected from Bangladesh Agricultural University and those of other five genotypes were collected from Bangladesh Agricultural Research Institute. Half strength MS medium supplemented with 20 mg/L sucrose was used for seed germination. For callus induction and shoot regeneration 5 different combinations each containing MS + 0.1mg/L NAA+ 2mg/L AgNO₃with different concentrations of BAP (T₁- 0 ppm, T₂- 1 ppm, T₃- 1.5 ppm, T₄- 2 ppm and T₅- 2.5 ppm) were used. For root initiation three treatments each containing half-strength MS medium supplemented with three different concentration of IBA (0 ppm, 0.1 ppm and 0.5 ppm) were used. The regenerated plantlets were transplanted from culture vessel to plastic pots containing 25% garden soil + 50% sand + 25% cowdung in growth chamber. For callus induction data were recorded on days to callus initiation, percent callus induction and nature of callus; for plantlet regeneration data were recorded on days to shoot initiation, number of callus showing shoots, total number shoot per Petri dish and for root initiation data were recorded on percent shoot showing root. The collected data were analyzed with proper statistical methods.

Source of Information: Pak. J. Bot., 41(3): 1233-1239, 2009

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that T₄ with the interaction of Tori-7 was the best performer for shoot regeneration. The regenerated plantlets were transplanted into plastic pots containing sterile soil, sand and cowdung in a 1: 2: 1 ratio for acclimatization. Gradually the plantlets were adapted to the soil.

Knowledge Management: Journal