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Eradication of Banana Bunchy Top Virus (bbtv) and Banana Mosaic Virus (bmv) from Infected Plant of Banana Cv. Amritasagar Through Meristem Culture

M. H. Kabir Shiragi
Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. A. Baque
Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

K. M. Nasiruddin
Professor
Department of Biotechnology, Bangladesh Agricultural University, Bangladesh

Abstract/ Executive Summary:

An experiment on virus free in vitro banana plantlets production by the meristem cultured from virus infected four month old banana suckers cv. Amritasagar were used as explants. Four levels of BAP (0, 3, 4 and 5 mg/l), 5 levels of NAA (0.0, 1.0, 1.5, 2.0 and 2.5 mg/l) were used as treatment for shoot proliferation in experiment-I and Four levels of IBA (0, 1, 2 and 3 mg/l), 4 levels of NAA (0, 2, 3 and 4 mg/l) were used for root formation in experiment-II. ELISA test was conducted for the confirmation of virus eradication rate of regenerated plants and survival rate of regenerated plants in experiment-III. A significant variation was found among the treatments. Studies on the different concentrations of BAP + NAA on shoot proliferation and differentiation, reveals that 4 mg/l BAP + 1.5 mg/l NAA produced the greater number of shoots (4.58/explant), the longest shoot (3.56 cm) and the highest numbers of leaves (3.11/explant). On the contrary, plantlets were cultured on half strength of MS media in different levels of IBA and NAA.The highest number of vigorous roots (6.98/explant) and maximum root length (5 cm) were recorded from the treatment combination 2 mg/l IBA+2 mg/l NAA and 3 mg/l IBA+ 3 mg/l NAA, respectively. While less vigorous root was found in control treatments. The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected plants was 57.14% and from banana mosaic virus (BMV) infected sources was 64.28%. The highest plant survival rate 83.33% was recorded in a mixture of sand, soil and cow dung (1:1:1). On the other hand, 67.67% plant survival rate was found in a mixture of coir and soil (1:2). Eradication of banana viruses from infected plants by meristem cultured is a useful tactic that will be helpful for commercial exploitation and virus free plant production for use of farmer’s level.

Keywords: Plants growth regulators, Meristem culture, Shoot & root proliferation, ELISA, Amritasagar

Location: Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

Start Date: 00-09-2003

End Date: 00-03-2004

Program Area: Crop-Soil-Water Management

Commodity: Banana

Objectives:

To find out the suitable concentrations and combinations of appropriate PGRs for the regenerations of virus free healthy planting materials of banana cv. Amritasagar.

Methodology:

The experiment on virus free banana plantlets production by meristem culture and establishment of virus free in vitro plantlets into rooting media was conducted at the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, during September 2003 to March 2004. Banana cv. Amritasagar was used as planting materials. MS Medium supplemented with different phytohormone as per treatments were used as culture medium. For the preparation of media, different stock solutions e.g. Stock solution I (Macronutrients), Stock solution II (Micronutrients), Stock solution III (Iron source), Stock solution IV (Vitamins and amino acids), Stock solution V (Growth regulators), hormone stock solution VI were prepared at the beginning and stored at 4±1°C. The culture vessels with media, glassware and instruments were then autoclaved for sterilization at 1.16-kg/cm² pressure at 121°C for 20 minutes. The medium was then cooled at room temperature before use. Initially, the culture room was cleaned by gently washing all floors and walls with detergent or Lysol (germicide). This was followed by carefully wiping them with 70% ethyl alcohol. The transfer area was also sterilized twice a month by 70% ethyl alcohol. Laminar Airflow Cabinet was usually sterilized by switching on the cabinet and wiping the working surface with 70% ethyl alcohol for 30 minutes before starting the transfer work.

Source of Information: South Pacific Studies Vol.29, No.1, 2008

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study indicated that to find out the suitable concentrations and combinations of appropriate PGRs for the regenerations of virus free healthy planting materials of banana cv. Amritasagar for use of farmer’s level through in vitro meristem culture that may be helpful for commercial exploitation.

Knowledge Management: Journal