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The Influence of Different Hormone Concentration and Combination on Callus Induction and Regeneration of Rauwolfia Serpentina L. Benth

U. Salma
Department of RDDR, Modern Herbal group, Dhaka - 1217, Bangladesh

M.S.M. Rahman
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh

S. Islam
Plant Tissue Culture Laboratory, BCSIR, Dhaka - 1205, Bangladesh

N. Haque
Department of RDDR, Modern Herbal group, Dhaka - 1217, Bangladesh

T.A. Jubair
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh

A.K.M.F. Haque
Biotechnology and Genetic Engineering Discipline, University of Development Alternative, Dhaka - 1207, Bangladesh

I.J. Mukti
Biotechnology and Genetic Engineering Discipline, University of Development Alternative, Dhaka - 1207, Bangladesh

Abstract/ Executive Summary:

The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L^-1 BA and 2.0 mg L^-1 NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L^-1 BA with 0.2 mg L^-1 NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L^-1 each of IBA and IAA.

Keywords: Propagation, Callus, Regeneration, Indole alkaloids, Endangered

Location: Plant Tissue Culture Laboratory, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka

Start Date: 00-00-2007

End Date: 00-00-2007

Program Area: Conservation and Biodiversity

Commodity: Plant(s)

Objectives:

To find out the influence of media composition on effective callus induction to facilitate modern approaches of alkaloids production as well as regeneration for the conservation of the threatened genotype.

Methodology:

The experiment was carried out in the year 2007 in the Plant Tissue Culture Laboratory, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka, Bangladesh. Nodal explants of R. serpentina from actively field grown plants were cleaned thoroughly under a continuous stream of running tap water for 30 min. After that the explants were washed with detergent and kept under running tap water for 15 min. Then treated with 1% Savlon and washed in running tap water for 30 min. Explants were surface sterilized with 50% (v/v) ethanol (1 min) and followed by 0.1% (w/v) HgCl₂ (3 min). Finally the explants were washed thoroughly three times with autoclaved double distilled water. The nodal explants were then cut into appropriate size (6-8 mm) and cultured on MS medium containing 1.5 mg L^-1 BA and 0.2 mg L^-1 NAA. Nodal pieces (6-8 mm) taken from 6-week-old in vitro shoots were used to initiate callus culture. In this study we report the influence of plant growth regulators such as BA and NAA for effective callus and shoot induction. Regenerated shoots were rooted on half strength MS medium with IBA and IAA. Media were adjusted to pH 5.8 before addition of 0.6% agar and autoclaved at 121 °C for 15 min. All cultures were incubated at 25 ± 1 °C with a photoperiod of 16 h at 3000 lux light intensity of cool white fluorescent light. Weekly visual observation of culture was made. All experiments were repeated twice with at least 18 cultures per treatment and data were taken after 4-6 weeks of culture.

Source of Information: Pakistan Journal of Biological Sciences, 11: 1638-1641; ISSN 1028-8880

Article Link (Online Journal): DOI: 10.3923/pjbs.2008.1638.1641; URL: http://scialert.net/abstract/?doi=pjbs.2008.1638.1641

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The study showed a higher root induction efficacy of 80% on half-strength MS medium supplemented with IAA and IBA combination. The root lengths were varied in all media concentrations.

Knowledge Management: Journal