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Proline Accumulation, Water Status and Chlorophyll Content in Leaf in Relation to Salt Tolerance in Soybean

M. A. Mannan
Department of Agronomy, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh.

M. A. Karim
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahaman, Agricultural University, Salna, Gazipur- 1706, Bangladesh.

Q. A. Khaliq
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahaman, Agricultural University, Salna, Gazipur- 1706, Bangladesh.

M. M. Haque
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahaman, Agricultural University, Salna, Gazipur- 1706, Bangladesh.

M. A. K. Mian
Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahaman, Agricultural University, Salna, Gazipur- 1706, Bangladesh.

J. U. Ahmed
Department of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh.

Abstract/ Executive Summary:

An experiment was conducted to study the effect of salinity on proline accumulation water status and chlorophyll content in teal of a salt - tolerant (AGS 313) and a salt - susceptible (Shohag) soybean (Glycine max L) genotypes. The plants were grown in pots inside a vinylhonse with 50 and 100 mM NaCI solutions and tap water as a control. Leaf proline accumulation, water uptake capacity (WUC) and concentration of chlorophyll a, b and total chlorophyll of the two genotypes were compared at 15, 30, 45 and 60- days after treatment imposition. Marked variation in accumulation of proline was found between the two genotypes differing in salt tolerance. Proline accumulation was increased with the Increasing salinity levels and with duration in both the genotypes. AGS 313 accumulated higher amount of proline under salt stress condition than that of Shohag at all the growth stages. Salinity also increased WUC in both AGS 313 and Shohag. The relative increase in WUC (% of the control) was greater in Shohag than that in AGS 313, which indicated that Shohag suffered more with water deficit than AGS 313. The chlorophyll a, b, and total chlorophyll in leaves of tbe two genotypes were decreased greatly with the increase in salt concentrations and with duration of exposure to salinity. The relative reduction (% of the control) of chlorophyll content was higher in Shohag than that in AGS 313. It was concluded that relatively higher amount of proline in AGS 313 had a greater osmoregulatory role in maintaining relatively lower WUC and higher leaf chlorophylls than those of Shobag.

Keywords: Chloropbylls, Proline, Salt tolerance, Soybean, Water uptake capacity

Location: Bangabandhu Sheikh Mujibur Rahman Agricultnral University, Salna, Gazipur, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Soybean

Objectives:

To study the effect of salinity on proline accumulation, water status and chlorophyll content in leaf of a salt - tolerant (AGS 313) and a salt - susceptible (Silohag) soybean (Glycine max L) genotypes.

Methodology:

The seeds of soybean (Glycine max L.) genotypes AGS 313 and Shohag were sown in plastic pots of 24cm x 30cm in size, containing 12 kg air dried soil, inside a vinylhouse under natural light at the Environmental Stress Research Site of Bangabandhu Sheikh Mujibur Rahman Agricultnral University (BSMRAU), Salna, Gazipur, Bangladesh. In a preliminary experiment the genotype AGS 313 was found more salt tolerant in terms of growth than the genotype Shohag. When the 1^st trifoliate appeared, 1000 ml of 0, 50 mM and 100 mM NaCI solutions were applied in each pot up to 60 days. Salt solutions were prepared by dissolving calculated amount of commercially available NaCI with tap water. Tap water was used as the control. The salt solution was applied stepwise with an increment of 25 mM in every alternate day till respective concentrations were attained. Treatment solutions were applied in excess so that the extra solution dripped out through the holes made at the bottom of the pots. Uppermost fully developed leaves were collected at 15,30,45, and 60 days after treatment imposition to measure proline, water uptake capacity and chlorophyll content. Four leaves from four separate plants in each treatment were considered for each measurement of the parameters. Water uptake capacity of leaf was measured using the following formula: Water uptake capacity (WUC)= (Turgid weight - Fresh weight/ Dry weight) ChIorophylls were estimated and expressed as mg g^-1 fw using the following equation: Chlorophyll a =[12.7 (D₆₆₃)-2.69 (D₆₄₅)] x [V/1000 x W], Chlorophyll b = [22.9 (D₆₄₅)- 4.68 (D₆₆₃)] x [V/1000 x W], Chlorophyll, (total) = [20.2 (D₆₆₃)- 8.02 (D₆₄₅)] x [V/1000 x W]

Source of Information: Indian J. Plant Physiol., Vol. 14, No. 2, (N.S.) pp. I30-I34 (April-June; 2009), ISSN 0019-5502

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The relative increase in WUC (% of the control) was greater in Shohag than that in AGS 313, which indicated that Shohag suffered more with water deficit than AGS 313. The chlorophyll a, b, and total chlorophyll in leaves of tbe two genotypes were decreased greatly with the increase in salt concentrations and with duration of exposure to salinity. The relative reduction (% of the control) of chlorophyll content was higher in Shohag than that in AGS 313. It was concluded that relatively higher amount of proline in AGS 313 had a greater osmoregulatory role in maintaining relatively lower WUC and higher leaf chlorophylls than those of Shobag.

Knowledge Management: Journal