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Assessment of Immune Response in Cattle Against Experimentally Prepared Trivalent (o, A, and Asia-1) Fmd Vaccine in Bangladesh

Md. Mashfiqur Rahman Chowdhury
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Md. Liakot Hossen
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Khaled Bin Amin
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

K. H. M. Nazmul Hussain Nazir
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Sultan Ahmed
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Marzia Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Mohammad Ferdousur Rahman Khan
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Md. Tanvir Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Md. Bahanur Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural Unviersity, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

This research work was conducted to investigate the effects of age, sex and breed on the induction of immune response against experimentally prepared inactivated trivalent (type O, A, and Asia-1) FMD vaccine. Twenty six cattle were divided into four test groups (Group A, B, C, and D; 5 cattle in each group) and one control group (n=6) based on breed (local and cross), age (≤12 months and >12 months), and sex (male and female). Test cattle were vaccinated with the experimentally prepared trivalent FMD vaccine. Pre- and post vaccinated sera from the vaccinated cattle were collected upto 63 days, and the sera were tested using liquid phase blocking enzyme linked immunosorbent assay (LPBE) that was specific for FMD serotypes O, A, and Asia-1. Antibody titers of all the pre-vaccinated serum samples were found to be under protection level. The females were found to be more protected (90%; n=9/10) as compared to males (70%; n=7/10). The titers obtained were statistically analyzed using t–test to observe the effects of age, breed and sex. It was observed that the mean values of antibody titer in cattle aging >12 months against O, A, and Asia-1 serotypes were significant (P<0.05) at 21, 49 and 63 days as compared to the values obtained from the cattle aging ≤12 months. In conclusion, the local female cattle aging >12 months showed better immune response towards trivalent FMD vaccine.

Keywords: Age, Cattle, ELISA, FMDV, FMD vaccine

Location: Mymensingh district, Bangladesh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Cattle

Objectives:

This study was undertaken with an objective to determine the impact of age, sex and breed to induce antibody response against experimentally prepared inactivated trivalent (O, A, and Asia-1) FMD vaccine in cattle.

Methodology:

Vaccine: The FMD vaccine was experimentally prepared as water-in-oil emulsion that contained partially purified inactivated FMDV strain (O, A, and Asia-1), Montanide and saponin. Three serotypes of FMDV had been isolated from field samples of different districts of Bangladesh during outbreak in May 2014, and the virus was adapted to grow in BHK-21 cell cultures. Sterility and safety of the vaccines are under-way; all the techniques followed the requirements described in OIE Manual (OIE, 2012). The vaccine batch was stored at 4-8ºC and used in vaccine efficacy trials subsequently. Experimental cattle: A total of 26 local and cross breed cattle aging between 6 to 24 months were randomly selected for this experiment. The cattle were reared by the farmers at their houses at Boyra, Mymensingh. The cattle were classified into four test groups (five cattle in each group) based on sex and breed; group A, B, C and D represented as local male, local female, cross male and cross female, respectively, whereas Group E was considered as non-vaccinated group (control; n=6). The experimentally prepared trivalent FMD vaccines were administered subcutaneously at neck region (dosed at 6 mL/animal) to the cattle of groups A, B, C and D, except control cattle. In vaccinated cattle, 11 animals aged ≤12 months, and 9 aged >12 months. There were no reports of clinical FMD from any of the experimental cattle before and during the course of the study. The cattle were reared under similar supervision and nutrition during trials. Ethical guidelines: For the animal experimentation, international as well as institutional guidelines were followed. Serum sample: About 3 mL of blood was collected without anticoagulant from the jugular vein of experimental cattle with the sterile syringe at 21, 35, 49 and 63 days post-vaccination. The syringes were placed in a slanting position at room temperature for clotting. After traction, syringes were placed in the incubator at 37ºC for 1 h to retract the clot properly. Then the sera were collected in sterile eppendorf tube. The tubes were centrifuged for 15 min at 1000 rpm to get more clear serum. The serum was then kept in sterile eppendorf tube and was preserved at -20ºC until used. ELISA test: Liquid phase blocking ELISA was carried out for the detection of FMDV antibodies in serum using FMD Antibody Detection Kit (BDSL, UK), as described by Hamblin et al. (1986 a, b). Statistical analysis: The data obtained were subjected to t-test used to analyze the effect of age, breed and sex in vaccinated cattle. Statistical analysis was done with SPSS 20 version.

Source of Information: J. Adv. Vet. Anim. Res., 2(4): 475-480, Volume 2 Issue 4 (December 2015)

Article Link (Online Journal): http://www.banglajol.info/bd/index.php/JAVAR/article/download/26097/17491

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results of the prevailing study indicate that induction of protective antibody level against FMD vaccine is delayed in cattle aging below 12 months of age as compared to the cattle aging older than 12 months. So, it can be concluded that age of cattle influence significantly to produce good immune response against FMD vaccine.

Knowledge Management: Journal