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Induced Breeding of Vietnames Koi (anabas Testudineus) Using Pituitary Gland (pg) and Ovaprim at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur

Md. Ruhul Amin
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Mamunur Rahman
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Sopna Aktar
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Badrul Alam
FDO, World Fish Centre, Uposhohor, Sylhet, Bangladesh.

Md. Ashraful Alam
Microbiologist, Jahanabad Sea Food Ltd., Khulna, Bangladesh

Abstract/ Executive Summary:

The research work was conducted for induced breeding of Vietnames Koi (Anabas testudineus) using carp PG extract and Ovaprim hormone at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur during March to May, 2015. Brood fishes were reared for 4-5 months in the brood rearing pond by providing farm-made artificial feed (containing 32% protein) for maturation before onset of breeding season. Two different hormones i.e., PG-PG (both for male and female), and PG-Ovaprim (PG for male and Ovaprim for female) were used as inducing agents for the purpose of induction of breeding A. testudineus. The broods were induced with PG-PG hormone at the doses of 2 mg PG/Kg body weight of male and 7 mg PG/Kg body weight of female and PG-Ovaprim hormone were used at the doses of 2 mg PG/Kg body weight of male and 7 ml Ovaprim/Kg body weight of female and designated as treatments T₁ and T₂, respectively. Ovulation rates (100%) were similar in both the treatments, whereas, fertilization, hatching, and survival rates were 78.11±3.24%, 70.22±3.40%, and 51.22±3.96% in T₁ and 71.00±3.97%, 60.89±4.20%, and 48.89±4.41% in T₂, respectively. Although comparatively better breeding performance was found for carp PG extract but these values were not significantly higher compared to Ovaprim. Therefore, comparing the breeding performance and considering the cost-benefit analysis it is suggested that Ovaprim could be used as an alternative of carp PG extract for the induced breeding of Vietnames koi.

Keywords: Induced breeding, Vietnames koi, Pituitary gland, Ovaprim

Location: Melandah, Jamalpur district, Bangladesh

Start Date: 00-03-2015

End Date: 00-05-2015

Program Area: Animal Health and Management

Commodity: Koi/Climbing

Objectives:

Objective of this study was to determine the effects of only PG both for male and female and PG for male and Ovaprim for female on the breeding performances i.e., fertilization, hatching and survival rates of Vietnames koi (A. testudineus).

Methodology:

The experiment was carried out at the Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur during the period from March to May, 2015. In this experiment, two types of tanks were used, one for conditioning of the brood fishes and another for spawning purpose. Both conditioning and spawning tanks were provided with continuous flow of water for sufficient oxygenation. Sexually mature brood fish were selected from two different ponds for avoiding inbreeding. One is from Mohalakhsmi Fish Hatchery, Bogra and another from own farm's pond. They were reared in cisterns and raceway system with water flow and fed 32% protein containing artificial feed at 5% body weight for two months. Before the breeding trial, the brood fish were collected by reducing the water of the raceway and the cisterns to avoid mechanical injury. Good and healthy broods were selected for breeding. Identification of male and female broods was done on the basis of some external features. The females could be easily recognized by their swollen abdomen and round and swollen urogenital papillae. On the other hand, the mature males were identified by their narrow abdomens and long protruded genital papillae. Sexually mature males and females weighing 180-200 g were selected for induced breeding. Selected broods were kept in cisterns with continuous water flow for about 6h for conditioning prior to injection. Twelve pairs of male and female broods were divided into two treatments and designated as T₁ and T₂ each with two replications having three pairs of broods. Broods were selected in such a way that the average weight of 3 females under each treatment remained approximately similar. Only carp PG extract was used both for male and female in T₁ and carp PG extract for male and Ovaprim for female was used in T₂. Male and female fishes were kept in separate cisterns and constant water flow was maintained to ensure proper aeration. The females under each treatment were kept separately in different cisterns. Freshly prepared extract of commercially available acetone dried PG was used for inducing ovulation in fish. To prepare the extract for injection, required amount of PG was carefully weighed out in an analytical balance. The required amount was calculated on the basis of the total body weight of all the fish of a particular treatment using the following formula: Weight of carp PG (mg) = Wb × Pt/100. Where, Wb represents total body weight of all the fish to be injected and Pt represents the rate in mg of PG to be injected/kg body weight under a particular treatment. The weighed PG was homogenized with a small volume of distilled water and the homogenate was carefully transferred to a centrifuge tube by using distilled water to ensure complete transfer. The mixture was centrifuged for 5 min at 5000 rpm. The clear supernatant was transferred to a vial and was made pre-determined volume with distilled water. On the basis of the body weight, 2 mg carp PG extract/kg male and 7 mg carp PG extract/kg female broods were used in T₁, where as 2 mg carp PG extract/kg male and 7 ml Ovaprim/kg female broods were used in T₁. In all cases hormones were administered intramuscularly to the fish on the dorsal side above the lateral line. After 10-12 h of hormone administration, females released eggs naturally and males also released their sperm naturally to fertilize the eggs. The fertilized eggs were incubated for 36-48 hrs in mini plastic circular incubator for hatching. For calculating percent fertilization, 50 eggs were taken from each group and number of fertilized and unfertilized eggs were counted under a microscope. The unfertilized eggs turned whitish and opaque few hours after incubation while the fertilized eggs remained transparent and showed distinct evidence of cell division of embryo when observed under microscope.

Source of Information: Asian J. Med. Biol. Res. 2015, 1 (3), 607-611; doi: 10.3329/ajmbr.v1i3.26484, ISSN 2411-4472

Article Link (Online Journal): http://banglajol.info/index.php/AJMBR/article/view/26484/17776

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In this experiment only carp PG extract at the dose of 2 mg/Kg body weight was used for all males in both the treatments and 7 mg carp PG extract and 7 ml Ovaprim per Kg body weight of female were used in T₁ and T₂, respectively. In T₁, the fertilization, hatching and survival rates were comparatively higher than T₂ but difference was not significant. At the same time the price of Ovaprim is almost less than half of the carp PG. Since no significant difference had been found on the breeding performance of Vietnames koi in response to the induction with carp PG and Ovaprim and as well as considering the cost-benefit analysis it might be concluded that hatchery operators should use ovaprim for induced breeding of Vietnames koi.

Knowledge Management: Journal