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Anti-fungal Potential of Tridhara (tridax Procumbens) Leaves

Md. Faruk Miya
Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Zakirul Islam, Sayeed Shahriyar
Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Riad Hossain Khan
Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Salim Reza
Department of Biotechnology, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The antifungal activity of methanolic extracts of leaves of Tridax procumbens was studied. Fungal strains Aspergillus niger and Aspergillus ocraceous were selected for the study. Disc diffusion method was applied against the selected fungal strains and compared to the drug ciprofloxacin to observe the antifungal activity of the methanolic extracts of Tridax procumbens leaves. The present study demonstrated that the disks of methanolic extracts of T. procumbens showed effective inhibition against A. niger and A. ocraceous compared to ciprofloxacin. The results of the current investigation suggest that the methanolic extracts of T. procumbens leaves can be used for the treatment of the diseases caused by the tested organism and it has significant scope to develop a novel broad spectrum of antifungal herbal formulations.

Keywords: Tridhara, Extracts, Medicinal plants, Leaves

Location: Jessore University of Science and Technology campus

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Extract (plant, seed)

Objectives:

To investigate the antifungal activity of ethanolic extract of leaves of T. procumbens (L) against Aspergillus niger and Aspergillus ocraceous comparing with ciprofloxacin.

Methodology:

The leaves of T. procumbens plant was collected from local area of Jessore University of Science and Technology campus region of Jessore. It was then botanically identified. The cleaned plant parts were then allowed for complete shade drying and then made to fine powder with a mechanical grinder and stored in an airtight container. The coarse powders from T. procumbens leaves were then subjected to successive extractions by methanol. The extraction was placed on a shaker at 40°C temperature for 24 hours for mild shaking. The crude extracts were then filtered by passing the extracts through filter paper and then concentrated under vacuum at 40°C by using a rotary evaporator. The residual extracts were stored in refrigerator at 4°C in small and sterile plastic bottles. Strains of the A. niger and A. ocraceous were obtained from Regional Agricultural Research Station. The fungus was grown at 27°C on potato dextrose agar (PDA). Spores of the fungus were collected from cultures on agar plates after 3 days as described. Potato is boiled with water for 30 minutes then 50ml potato extract took in a conical flask with 1gm glucose and P^H was adjusted to 5.6 for growing fungus. Finally 1gm agar was added in the conical flask. 100 mg of the leaf extract were accurately weighed by the electronic balance and taken in small volumetric flasks. Then 1600 µl of methanol was added and triturated in unidirectional manner using a vortex mixer. So, these samples were prepared by addition of solvent calculatively. Three types of discs were used for antibacterial screening: 1. sample discs, 2. standard discs and 3. blank discs. Each potato dextrose agar (PDA) plates were divided into four portions i.e. two for samples one for standard (positive control) and one for blank (negative control). Sterile filter paper discs (5 mm in diameter) were taken as sample discs were placed on surface of the media at accurate positions. 10 µl of the test sample from 25 µg/µl and 50 µg/µl solutions were applied on the discs aseptically under the laminar air flow to get concentration of 250 µg and 500 µg per disc respectively for each sample. Sterile filter paper discs (5 mm in diameter) were taken as sample discs. 10 µl methanol was applied on the blank discs as negative control. They ensure that the residual solvent’s activity and the filter paper were not active themselves. Ciprofloxacin (5 µg/ disc) antibiotic discs were used as positive control to ensure the activity of standard antibiotic against the test organisms as well as for comparison of the response produced by the known antimicrobial agent with that produced by test samples. Ciprofloxacin (5 µg/ disc) was placed on the labeled portion of the petridish. Finally the plates were incubated upside down at 37°C for 16-18 hours.

Source of Information: Asian J. Med. Biol. Res. December 2015, 1(3): 686-689, ISSN 2411-4472

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/ajmbr.v1i3.26497

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The extracts of T. procumbens were found to be effective antifungal agents against human pathogens. This study paves the way for further attention and research to identify the active compounds responsible for the plant biological activity with the required minimum zone of inhibition. Further studies should be undertaken to elucidate the exact mechanism of action by which extracts exert their antifungal effect to identify the active ingredients which can be used in drug development program for safe health care services. T. procumbens is a very commonly used medicinal plant for wound healing and other inflammations even in eyes and can be used for the development of new drug formulation for treating bacterial and fungal infections and also for the elimination of the concern of microbial resistance.

Knowledge Management: Journal