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Laboratory Bioassay of Four Fungicides Against Mycelial Growth of Alternaria Brassicae

M.N. Haider
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

M.R. Islam
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

F.M. Aminuzzaman
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

H. Mehraj
Department of Horticulture; Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

A F M Jamal Uddin
Department of Horticulture; Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted at the Seed Health Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the bioassay of fungicides in controlling mycelial growth of Alternaria brassicae in-vitro. In this experiment, four fungicides viz. Rovral 50 WP @ 0.2%, Dithane M-45 @ 0.45%, Ridomil gold MZ-72 @ 0.2%, Bavistin DF @ 0.1% and control (sterile water) were used for the assessment. Rovral 50 WP @ 0.2% was found effective to control the mycelial growth of Alternaria brassicae. Minimum mycelia growth (1.9 cm) was found with Rovral 50 WP @ 0.2% whereas maximum from control (6.0 cm) at 15 DAI. On the other hand, Rovral 50 WP reduced 68.9% mycelial growth of Alternaria brassicae.

Keywords: Alternaria brassicae, Bioassay, Fungicides

Location: Seed Health Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka

Start Date: 00-11-2012

End Date: 00-02-2013

Program Area: Pest Management

Commodity: Mustard

Objectives:

To determine effectiveness of the fungicides on radial mycelial growth of A. brassicae.

Methodology:

Experiment was conducted at the Seed Health Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Recommended doses of fungicidal solution were prepared by mixing thoroughly with requisite quantity of chemical and normal water. It was required 2.0 gm/liter of Rovral 50 WP, 4.5 gm/liter of Dithane M-45, 2.0 gm/liter of Ridomil gold MZ-72 and 1.0 gm/liter of Bavistin DF for preparation of solution with respective concentration. An in-vitro test was conducted to determine effectiveness of the fungicides on radial mycelial growth of A. brassicae. For the laboratory experiment the plates were arranged on the laboratory desk following Complete Randomized Design (CRD) with five replications. Isolation and identification of pathogens from leaf tissue: Plant material infected with Alternaria brassicae was sampled randomly from Brassica species cultivated field in Sher-e-Bangla Agricultural University. Infected leaves were collected for sampling. Diseased leaves were cut into pieces (4.0 mm diameter) and surface sterilized with HgCl₂ (1:1000) for 30 seconds. Then the cut pieces were washed in sterile water thrice and then placed onto acidified PDA in petridish. The plates containing leaf pieces were placed at room temperature for seven days. When the fungus grew well, and sporulated, then the slide was prepared from the PDA and was identified under microscope with the help of relevant literature. Cup/Groove method for the bioassay of fungicides against Alternaria brassicae: From a PDA plate three 5.0 mm discs of the medium were scooped from three places maintaining an equal distance from the centre by a sterilized disc cutter. One milliliter of fungicides solution was put into each hole and the plates were stored overnight in refrigerator for diffusion of the input in the medium around the hole before resumption of fungal growth. The next day, one 5 mm culture block of Alternaria brassicae was cut and placed at the centre of the treated PDA plate. There were five treatments on the experiment viz. Rovral 50 WP, Dithane M-45, Ridomil gold MZ-72, Bavistin DF and control. Each treatment was replicated five times. For control treatment, only sterile water was used instead of fungicides. The plates were then placed at 25±1°C for 15 days. The linear growth (cm) of mycelium of Alternaria brassicae was recorded at 3 days interval until the control plates were filled in. The data were statistically analyzed using MSTAT-C com

Source of Information: International Journal Of Business, Social And Scientific Research, Page: 06-08, Volume 01, Issue 01, (2013)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Rovral 50 WP reduced the highest radial mycelial growth (68.91%) of Alternaria brassicae followed by Dithane M-45 (63.87%), Ridomil gold MZ-72 (55.46%) and Bavistin DF (50.42%) compared to control. However further investigation is needed to test the efficacy of the Rovral 50 WP.

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