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Kinetin (kin) and Indole-3- Acetic Acid (iaa) on in Vitro Shoot and Root Initiation of Tuberose

M. R. Ali
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

H. Mehraj
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

A. F. M. Jamal Uddin
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted at the Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka Bangladesh from July 2011 to September 2012 to evaluate the effect of different concentrations of KIN and IAA on In vitro shoot and root initiation of tuberose. Experiment consisted seven treatments viz. T₁: Simple MS, T₂: MS+0.5 mg/L, T₃: MS+1.0 mg/L, T₄: MS+1.5 mg/L, T₅: MS+2.0 mg/L and T₆: MS+2.5 mg/L for both KIN and IAA following Completely Randomized Design with five replications. Early shoot initiation (10.1 days), leaf initiation (12.2 days), root initiation (14.92 days) was found from T₃. On the other hand, maximum number of leaves (3.00/plantlet), longest leaves (5.48 cm) and maximum number of roots (3.05/plantlet) was found from T₃ at 60 DAI.

Keywords: KIN, IAA, Shoot, Root and Tuberose

Location: Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka

Start Date: 00-07-2011

End Date: 00-03-2012

Program Area: Crop-Soil-Water Management

Commodity: Tube rose

Objectives:

To evaluate the effect of different concentrations of KIN and IAA on In vitro shoot and root initiation of tuberose.

Methodology:

An experiment on in vitro regeneration of tuberose was conducted in the Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during July 2011 to March 2012. Disease free bulbs of tuberose were collected from Bangladesh Agricultural Research Institute (BARI), Gazipur for explants. Murashige and MS medium were used with different hormonal supplements as culture medium for shoot and root initiation. The culture tube containing medium was sterilized in an autoclave at temperature of 121°C for 20 minutes at l5 psi pressure. The medium was then cooled at room temperature before use. The lead of cabinet then was closed well and UV was switched on while turning off the air flow. The UV light of cabinet was left on for 30 minutes and the surface area is wiped with 70% ethanol. The temperature of culture room was maintained within 25±1°C with the help of air conditioner with 16 hours photoperiod and 3000 lux light intensity by using white florescent lights. Buds were first sterilized with 70% (v/v) ethanol for few seconds after that surface sterilized by immersing in 0.1% HgCl₂ solution for 4-5 minutes and then was washed several times with sterilized distilled water. Then the bud was cut aseptically into small pieces with the help of sharp aseptic knife and the small segments of bud were used as explants. Three to five segments of explants were directly inoculated into each test tube containing 10 ml of MS medium supplemented with different callus induction hormonal combinations of IAA and KIN. Single factorial experiment consisted seven treatments viz. T₁: Simple MS, T₂: MS+0.5 mg/L, T₃: MS+1.0 mg/L, T₄: MS+1.5 mg/L, T₅: MS+2.0 mg/L and T₆: MS+2.5 mg/L for both KIN and IAA following Completely Randomized Design with five replications. Data were taken on days to shoot initiation, days to leaf initiation, days to root initiation, number of leaves/plantlet, length of the leaves/plantlet, number of roots/plantlet and length of roots/plantlet (cm). The collected data on different parameters were analyzed using a MSTAT-C package computer program. The analysis of variance was performed and means were compared by Duncan Multiple Range Test (DMRT) at 5% level of significance.

Source of Information: Int. J. Sustain. Agril. Tech. 10(8): 01-04, (2014)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Addition of 1.0 mg/L KIN and IAA with MS medium was best for the shoot and root initiation respectively in tuberose.

Knowledge Management: Journal