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Performance of Seed Health Quality and the Effect of Anthracnose Disease in Germination Under the Laboratory in Cvl-1 Jute Seeds Collected from Different Sources of Bangladesh

Nazmul Hasan
Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh.

Kohinoor Begum
Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh.

Rabeya Parvin
Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh.

Sanjida Khandker
Department of Agricultural Extension, Ministry of Agriculture, Laxmipur, Bangladesh.

Rafiqul Islam
Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh.

Abstract/ Executive Summary:

Six hundred CVL-1 jute seed samples were collected from different sources in Bangladesh and were subjected to health test. Seed samples were categorized on the basis of presence of Colletotrichum corchori as 0, 5, 10, 15, 20 and 25%. Germination of the collected seed samples found to be varied significantly. The highest germination (96.00%) was recorded in breeder seeds and the lowest (67.33%) was in farmers’ seeds. In seedling symptom test, the highest (96.00%) and the lowest germination (73.00%) were recorded in case of 0% and 25% initial seed borne infection of C. corchori, respectively. Seeds having higher level of seed infections with the pathogens caused reduction of seed germination. Lower germination was recorded at higher prevalence of initial seed borne infection by C. corchori.

Keywords: Seed borne infection, Biotic stress, Seed health test, Seedling symptom test, Colletotrichum, Incidence

Location: Seed Pathology Laboratory at Plant Pathology Department, Pest Management Division, Bangladesh Jute Research Institute.

Start Date:

End Date:

Program Area: Pest Management

Commodity: Diseases, Jute

Objectives:

To observe the health quality and effect of Colletotrichum corchori on germination under laboratory condition.

Methodology:

The experiment was conducted in Seed Pathology Laboratory at Plant Pathology Department, Pest Management Division, Bangladesh Jute Research Institute. The variety CVL-1 belonging to deshi pat ( Corchorus capsularis ) of different seed tires was used for conducting the experiments. Altogether 600 seed samples were collected from different locations of Bangladesh of which there were 15 breeders, 5 foundation seeds, 7 certified seeds and 573 farmers’ seeds. Seed samples were obtained from the seed lots of each tier. Primary seed sample of 50g were randomly taken from 10 different positions of the seed lot. All the primary seed samples were mixed thoroughly to make a composite sample. Thus each composite sample was 500 g of seeds. As the size of each composite sample was 500 g, so it was regarded as submitted sample. The submitted seed samples were kept in plastic container. All the seed samples were labeled properly and preserved in Gene Bank of BJRI at 5°C till the samples were used for conducting respective research. Working seed samples were taken from the preserved seed samples as per requirement. Total procedure was maintained following the Rules of ISTA. Following the rules of ISTA, four hundred seeds that were taken randomly from the well-mixed seed sample [10]. The working samples were divided into four replications and thus one replication contained 100 seeds. To ensure adequate spacing, 100 seeds were divided into four sub replications and each sub replication contained 25 seeds. The seeds were germinated on top of three layers of Whatman no.1 filter paper. The filter papers were soaked in water and placed at the bottom of 9-cm diameter plastic petridish and thereafter 25 seeds were placed on the top of filter paper. Thus 400 seeds were placed in 16 replicate of petridishes. Evaporation of water was minimized by tightly fitting the lids of the petridishes. The petridishes were placed inside the incubator maintaining the temperature at 30°C for five days. Seeds producing both plumule and radical after incubation were counted as germinating seeds and expressed as percentage. Six hundred seed samples, of variety CVL-1, were collected from different locations of Bangladesh for health analysis for detection of C. corchori. Seed health analysis was conducted by blotter method following the International Rules for Seed Health Testing. In this method 9 cm diameter plastic petridish and locally packed Whatman no. 1 filter paper were used. Two hundred seeds were taken randomly and placed on the moist filter paper in eight replicate petridished. The petridishes with seeds were then incubated at 22±2°C for seven days in the laboratory. After incubation of the seeds was examined under stereomicroscope and the pathogen, C. corchori were identified by following the key of Sutton. Collected seed samples from all seed tires were categorized on the basis of presence of Colletotrichum corchori as 0, 5, 10, 15, 20 and 25% infection with C. corchori for conducting further experiment. Nature and rate of transmission of C. corchori were determined by water agar test tube seedling symptom test. In this technique 6 ml 1% water agar were used in each glass test tube (2.0 cm diameter X 15 cm in length) and sterilized in autoclave. Highest percentage of pure infection was used for the study. Laboratory experiment data were analyzed following Completely Randomized Design (CRD). Mean comparisons among the treatments were compared by Duncan’s Multiple Range Test.

Source of Information: World Journal of Agricultural Sciences 11 (4): 210-214, 2015, ISSN 1817-3047

Article Link (Online Journal): DOI: 10.5829/idosi.wjas.2015.11.4.1858

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The highest germination was recorded in breeder seeds and the lowest was in farmers’ seeds in the performance of seed health quality test. The highest seed borne infection with the lowest germination was recorded in farmers’ seed and the lowest seed borne infection with the highest germination was recorded in breeder seed. In seedling symptom test the highest germination was recorded in case of 0.00% and the lowest germination was recorded in case of 25.00% initial seed borne infection of C. corchori. The highest post emergence infection, germination failure and total death were recorded in case of 25.00% and no post emergence infection, germination failure and total death were recorded in case of 0.00% initial seed borne infection of C. corchori. Findings on the incidence of anthracnose after sowing of seeds with different initial % infections of C. corchori at laboratory reveals that all the samples had unsuccessful germinating trend with the increase initial % infection.

Knowledge Management: Journal