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Bacteriological Analysis of Some Commercially Packed and Fresh Fruit Juices Available in Jessore City: a Comparative Look

Md. Munjur Kader
Department of Microbiology, Jessore University of Science and Technology, Jessore, Bangladesh

Abdullah Al Mamun
Department of Microbiology, Jessore University of Science and Technology, Jessore, Bangladesh

Md. Tanvir Islam
Department of Microbiology, Jessore University of Science and Technology, Jessore, Bangladesh

Nigarin Sultana
Department of Microbiology, Jessore University of Science and Technology, Jessore, Bangladesh

Abstract/ Executive Summary:

Fruit juices are considered as the most consumed non-alcoholic beverages throughout the world to all age groups. In general, microbial growth in fruit juices is prohibited by using preservatives and also through other environmental factors during production. Still, there are several reports of illnesses due to the food borne diseases associated with the consumption of fruit juices at several places around the globe. So, from the point of view of public health, it is quite important to assess the microbiological quality of both fresh and commercially packed fruit juices available for the consumers. Present investigation attempted to resolve the microbiological attributes of the fruit juices collected from different areas around Jessore city. Ten fresh fruit juices and ten commercially packed fruit juices were collected. Standard plate count techniques were followed to assess total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) on different culture media. Samples were found to harbor viable bacteria within the range between 10³-10⁸ cfu/ml. 19 samples exhibited the presence of staphylococcai. Total coliforms were detected in 17 samples within the range of 10³-10⁶ cfu/ml which were further detected as Escherichia coli, Klebsiella spp. and Enterobacter spp. From all the assessment, the study demonstrates that the quality of both packed and fresh juices was unsatisfactory and hence the products need to be microbiologically controlled in order to ensure the overall health safety.

Keywords: Fruit juices, Public health, Total coliform count, Escherichia coli

Location: Department of Microbiology, Jessore University of Science and Technology, Jessore

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Juice

Objectives:

To compare the quality of fresh and commercially available fruit juices by assessing their microbial load and the presence of pathogenic bacteria.

Methodology:

Collection of samples - Twenty samples were collected, of which 10 fresh juice samples were collected from different locations of Jessore city and other 10 juice samples were commercially packed juices available in the market. Enumeration of total bacteria - 1 ml of sample was transferred with a sterile pipette to the tube containing 9 ml of normal saline to make 10-1 dilution. In such a way, dilution up to 10-7 was made. 0.1ml of each dilution was evenly spread on the nutrient agar medium and incubated at 37^oC for 24 hours. Plates were screened for the presence of discrete colonies after incubation period and the actual numbers of bacteria were estimated as colony forming unit per ml (cfu/ml). Quantitative analysis for the presence or absence of specific microorganisms was done by plating on selective media. Total coliform count (TCC) and total staphylococcal count (TSC) were performed in similar manner as described above using MacConkey agar and Mannitol Salt Agar (MSA) medium, consecutively. Estimation of bacterial load was performed by standard method. The microbiological condition of safety and hygiene were then assayed by comparing the obtained results with the limit of Gulf standard, known as the recommended microbiological standard for fruit juices in the Gulf region. Identification of coliform bacteria - For presumptive identification of coliform bacteria isolates were randomly selected for major biochemical tests such as Triple Sugar Iron (TSI), Motality Indole Urease (MIU), Methyl-Red (MR), Voges-Proskaure (VP), Citrate Utilization, Catalase test and Oxidase tests. The tests were carried out following the standard methods. Isolates were then identified according to the Bergey’s manual of determinative bacteriology and manual for the identification of medical bacteria.

Source of Information: International Journal of Biosciences | IJB |; ISSN: 2220-6655 (Print) 2222-5234 (Online); Vol. 5, No. 1, p. 415-420, 2014

Article Link (Online Journal): http://www.innspub.net; http://dx.doi.org/10.12692/ijb/5.1.415-420

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the data presented in the current study, it could be hard to claim that, consumption of commercially packed juice was safe than fresh juices; because almost all types of fresh and commercially packed juice samples collected from different areas of Jessore city were not satisfactory as Escherichia coli, Klebsiella spp., Enterobacter spp., and Staphylococcus spp., were detected in large numbers from samples.

Knowledge Management: Journal