The samples of 11 categories of flowers including Hibiscus rosa-sinensis (Jaba), Ixora coccinea (Rangan), Ipomoea digitata (Giant potato), Allamanda cathartica (Allamanda), Nymphaea nouchali (Water Lily), Vinca rosea (Nayantara), Rosa kordesii, Gladiolus hybrid, Acmella oleracea (Toothache plant), Nyctanthes arbor-tristis (Shiuli) and Pseudomussaenda flava were randomly collected during August 2013-October 2013 following standard protocol (18). Microorganisms were isolated and identified. In the current study, the pathogenic isolates were examined for their antibiotic susceptibility traits by disc diffusion assay on Mueller-Hinton agar (Difco, Detroit, MI) against commonly used antibiotics following the standard protocol (19, 20). Antibiotics used in the study included erythromycin (ERY, 15 µg), amoxicillin (AMO, 30 µg), ceftriaxone (CEF, 30 µg), ciprofloxacin (CIP, 5 µg), streptomycin (SET, 10 µg), ampicillin (AMP, 10 µg), tetracycline (TE, 30 µg), chloramphenicol (CHL, 30 µg), cefixime (CFM, 5 µg), gentamycine (GEN, 10 µg), azythromycin (AZI, 15 µg) and cefalexim (CFL, 30 µg). According to the Clinical and Laboratory Standards Institute (CLSI) guidelines, the anti-bacterial activity of flower samples was studied through broth micro-dilution method (5, 21, 22). In order to determine the MIC of the flower samples, 100 µL of bacterial inoculam was prepared with final concentration of 10 5 cfu/mL and introduced into appropriately labeled sterile tubes containing MH broth. In current study, different volume of samples ware employed (16 µL, 32 µL, 64 µL, 128 µL, 256 µL, 512 µL, 1024 µL and 2048 µL) into the MH broth to find out the lowest concentration, which can trim down the maximum number of bacterial cell (5).