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Research Detail

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Khandker Shazia AFRIN
Sher-e-Bangla Agricultural University, Department of Genetics and Plant Breeding, Dhaka-1207, Bangladesh

Firoz MAHMUD
Sher-e-Bangla Agricultural University, Department of Genetics and Plant Breeding, Dhaka-1207, Bangladesh

Md. Shahidur Rashid BHUIYAN
Professor
Sher-e-Bangla Agricultural University, Department of Genetics and Plant Breeding, Dhaka-1207, Bangladesh

Md. Abdur RAHIM
Sher-e-Bangla Agricultural University, Department of Genetics and Plant Breeding, Dhaka-1207, Bangladesh

A field experiment was conducted with 22 Brassica napus L. advanced lines at the experimental farm of Sher-e-Bangla Agricultural University, Dhaka from November 2008 to March 2009 to asses genetic diversity among advanced lines of Brassica napus L.. Different multivariate analysis techniques were used to classify 22 Brassica napus genotypes. The genotypes were grouped into four clusters. The highest inter-cluster distance was observed between clusters II and IV whereas the maximum intra-cluster distance was found in cluster II. Therefore, the genotypes belonging to cluster I and cluster II, cluster II and cluster III and cluster III and cluster IV have been selected for future hybridization program. The role of number of secondary branches per plant and number of siliqua per plant in both vectors were important components for genetic divergence in these materials. Considering group distance and other agronomic performance the inter-genotypic crosses between G1 and G2, G2 and G6; G6 and G7; G6 and G8 and G7 and G8 might be suggested for future hybridization program.

  Genetic, Divergence, Cluster, Crosses, PCA, CVA, Brassica napus
  Experimental farm of Sher-e-Bangla Agricultural University, Dhaka
  00-11-2008
  00-03-2009
  Variety and Species
  Mustard

Objective of the study was to asses genetic diversity among advanced lines of Brassica napus L..

The advanced lines of Brassica napus L. for the experiment were collected from the Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University (SAU) and three released varieties collected from Bangladesh Agricultural Research Institute (BARI) as presented. The experiment was laid out in Randomized Complete Block Design (RCBD). The field was divided into three blocks; the blocks were sub-divided into 22 plots where genotypes were randomly assigned. The plot size was 3 m length with two rows. Row to row and plant to plant distances were 25 cm and 15 cm, respectively. Seeds were sown in lines in the experimental plots on 12 November, 2008. Adequate soil fertility was censured by applying of Urea, TSP, MP, Gypsum and Borax at 250-170-85-150-5 kg/ha, respectively. Urea was applied by two installments. Total amount of TSP, MP, gypsum and borax along with half of the urea were applied at the time of final land preparation as a basal dose. The second half of the urea was top-dressed at the time of flower initiation. Standard Intercultural operations (such as weeding, thinning, irrigation, pest management, etc.) were done uniformly in all the plots to ensure proper growth condition for the crop. Harvesting was started from February 20, 2009 depending upon the maturity. Observations were recorded on 10 randomly chosen plants from each plot. Data were collected on Days to 50% flowering, Days to 80% maturity, Plant height (cm), Number of primary branches per plant, Number of secondary branches per plant, Siliqua length (cm), Number of siliqua per plant, Number of seeds per siliqua, 1000 seeds weight (g) and Seed yield per plant (g). Multivariate analysis viz., Principal Component Analysis (PCA), Principal Coordinate Analysis (PCO), Cluster Analysis (CLU) were done by using GENSTAT 5 Release 4.1 (PC/Windos NT) soft ware program.

  Agriculturae Conspectus Scientificus . Vol. 77 No. 2 p- 81-85 , (2012)
  
Funding Source:
1.  Government Budget:  
  

Considering the magnitude of genetic distance, contribution of character towards divergence, magnitude of cluster mean and agronomic performance the following genotypes were promising: G1 for higher number of seeds per siliqua and long siliqua, G2 for higher number of secondary branches per plant, tallness, higher number of siliqua per plant and seed yield per plant, G6 for earliness, G7 for larger seeds, and G8 for higher yield per plant. Therefore considering group distance and other agronomic performance the inter-genotypic crosses between G1 and G2; G2 and G6; G6 and G7; G6 and G8, and G7 and G8 might be suggested for future hybridization program.

  Journal
  


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