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Phytochemical Screening of Catharanthus Roseus and Ficus Racemosa Leaves Extracts: a Statistical Inference

Shohel Hossain
Biomedical Research Laboratory, Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh.

Masum Hossain
Biomedical Research Laboratory, Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh.

Ziaul-Haque
Biomedical Research Laboratory, Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh.

M Moyen Uddin PK*
Biomedical Research Laboratory, Department of Biochemistry, Primeasia University, Dhaka-1213, Bangladesh.

Abstract/ Executive Summary:

This study was aimed at conferring the comparative abundances of ten different phytochemicals (alkaloids, polyphenols, flavonoids, tannins, saponins, sterols, vitamin-C, coumarins, terpenoids, and cardiac glycosides) from leaves of Catharanthus roseus and Ficus racemosa. The color strength or the precipitate formation was used as analytical answers to these tests. Seven phytochemicals viz. alkaloids, polyphenols, tannins, saponins, sterols, terpenoids, and cardiac glycosides in Catharanthus roseus leave {X² (df); p-value: 6.2 (2); 0.04} whereas saponins in Ficus racemosa leave {X² (df); p-value: 3.8 (2); 0.15} were also acknowledged as highest concentration category in this study. It is anticipated that the vital phytochemical properties documented in our study in the native medicinal plants of Catharanthus roseus and Ficus racemosa will be beneficial for explanation and groundwork of Pharmacognosy profiling of medicinal plants.

Keywords: Cassava, Medicinal plants, Statistical inference, Phytochemicals

Location:

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The specific objective was to statistical inference for the distribution of ten selected phytochemicals within and/or among nominated plants leaves.

Methodology:

Plant materials: The present study encompassed plant species which were Catharanthus roseus and Ficus racemosa. Chemicals: 80% Methanol, H₂S0₄, Wagner’s reagent (Iodine in potassium iodide), NaOH, HCl, FeCl₃, [K3 (Fe (CN) 6], Acetic anhydride or Acetyl acetate, Chloroform, NH₄OH, Ether and Indophenols. Sample collection: Two medicinal plants were collected locally from the farm lands in Bangladesh. Catharanthus roseus and Ficus racemosa leaves were collected from Tongi (Gazipur) and Chadpur local area in Bangladesh respectively. The plants collected were identified by local agriculture officer. Fresh and tender leaves of selected plants were used for phytochemical analysis. Preparation of plant extract and phytochemical screening: The leaves of the selected plants were collected from the plants and then washed off under running tap water to eradicate dust. The plant samples were then air dried for few days and the leaves were grinded off into powder and kept in polythene bags for future uses. The extracts of selected sample powder were prepared by soaking 50gm of dried powder in 100 ml 80% methanol and shaken well. The solution then filtered with the help of filter paper and filtered extracts of the selected plant sample were taken and used for further phytochemical analysis. Phytochemicals screening of the selected plant extracts were done by using standard methods. Statistical analysis: The screening of ten selected phytochemicals among the nominated plants Catharanthus roseus and Ficus racemosa leaves was statistically evaluated. Pearson Chi-square test was used to find out correlation of phytochemicals distribution in selected plants leaves extracts. This analysis was carried out using SPSS version 21.

Source of Information: Int. J. Bioassays, 2015, 4 (01), 3606-3610 ISSN: 2278-778X

Article Link (Online Journal): file:///C:/Users/barc/Downloads/615-778-1-SM.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The selected two medicinal plants are the source of the phytochemicals play a vital role in preventing various diseases. The phytochemical analysis of the medicinal plants is also important and has commercial attention in both research institutes and pharmaceutical companies for new drugs manufacturing. The important phytochemical properties acknowledged by this study in the local plant of Catharanthus roseus and Ficus racemosa leaves will be helpful in the managing different diseases. So, the need to discover and develop Catharanthus roseus and Ficus racemosa plant is crucial, especially in the view of the rapidly growing need for improvement in the medicine. More so, the plant, if developed can be of immense use to both pharmaceutical and cosmetic industries, since it contains bioactive compounds.

Knowledge Management: Journal