Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Occurrence of Anthracnose Disease Incidence and Its Severity on Guava Seedlings at Different Locations of Bangladesh

M.N. Islam
Scientific Officer
Plant Pathology Division, BARI, Bangladesh

M.S.M. Chowdhury
Professor
Plant Pathology Department, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M.M. Rahman
Scientific Officer
(Plant Pathology) Breeder Seed Production Centre, BARI, Bangladesh

M.M. Alam
Senior scientific officer
Plant Pathology Division, BARI, Bangladesh

Abstract/ Executive Summary:

Guava, a major fruit crop of Bangladesh, was surveyed during the period of July, 2010 to April, 2012 for the incidence of anthracnose disease and its severity. The study was carried out to observe the effect of temperature, relative humidity and rainfall on the incidence of anthracnose disease and its severity at seedling stage. The causal pathogen of anthracnose, Colletotrichum gloeosporioides was identified and its incidence and severity on guava at seedling stage was studied. A significant variation in development of the disease was observed in varied weather parameters. Occurrence of seedling anthracnose disease was positively correlated with temperature, rainfall and relative humidity. Therefore there were great variation of disease incidence and its severity on guava was evident from one location to another and season to season. These weather parameters should be critically addressed for the host-pathogen interaction and to find out the most appropriate time for combating the disease at minimum cost.

Keywords: Anthracnose, Colletotrichum gloeosporioides, Disease incidence, Guava and severity

Location: Eight nurseries of Dhaka, Gazipur, Barisal and Khagrachari districts.July, 2010 to April, 2012

Start Date: 00-07-2010

End Date: 00-04-2012

Program Area: Pest Management

Commodity: Guava

Objectives:

To observe the effect of temperature, relative humidity and rainfall on the incidence of anthracnose disease and its severity at seedling stage.

Methodology:

Location of survey area: Prevalence of diseases incidence on guava seedlings raised in the selected nurseries were surveyed. The study was carried out in eight nurseries of Dhaka, Gazipur, Barisal and Khagrachari districts. The eight nurseries of four districts were surveyed: Dhaka (Green orchid nursery, Agargaon and Barisal nursery, Savar); Gazipur (Gazipur nursery and Laxmipur nursery); Barisal (Sarchina nursery and Riyad nursery) and Khagrachari (Ramghor Hill Research Center and Ramghor nursery). Observation of the symptoms: Symptoms of the diseases were studied by visual observation. Sometimes hand lens was used for critical observation of the disease and sometimes a disease was identified based on matching the observed symptoms in the infected plants with the symptoms published in Ber and other guava disease compendium. Identification of causal organisms Collection of diseased specimen: Diseased leaves were collected from the infected plants representing the different areas of survey. The specimens were preserved in the laboratory following standard procedure of preservation of disease specimens until isolation was made. Isolation of causal organisms was made by two methods as follows: Moist blotter method: The pathogen associated with the diseased plant parts (leaves) were cut into pieces by scissors and placed on the moist filter paper (Whitman no.1). Three pieces of filter paper were moistened by dipping in sterile water. The Petri dishes with the diseased specimens were incubated at 22±2°C under 12/12 alternating cycles of NUV and darkness in the incubation room of the Seed Pathology Lab (SPL) for three to five days. After incubation the plates were examined under stereomicroscope for primary identification of the organisms (fungi). The fungi were transferred to PDA plates for proper sporulation and purification. Agar plate method: The diseased plant parts (leaves) were surface sterilized by dipping them in 0.001% HgCl₂ solution for 1.5 minutes and washed three times with sterile water and there after placed on PDA (Potato = 200 g, Dextrose = 17 g, Agar = 17-20 g, Water = 1000 ml) plates aseptically. The plates were incubated at 28°±1°C for 15-20 days and examined daily for any fungal growth. Epidemiology of disease incidence and severity Survey period: Altogether eight surveys were made during the period from July, 2010 to April, 2012. Where First, second, third, fourth, fifth, sixth, seventh, and eighth surveys were made in July, 2010; October, 2010; January, 2011; April, 2011; July, 2011; October, 2011; January, 2012; and April, 2012, respectively. Data collection during survey: During the survey in the nurseries, total numbers of guava seedlings as well as number of diseased seedlings in the nurseries were recorded. Then 30 seedlings were randomly selected for counting diseased leaves and disease free leaves. Moreover, five leaves per plant were randomly selected to determine the disease severity. Determination of disease incidence and disease severity: For calculation of disease incidence every seedling was counted in the nursery and also counted the infected seedlings and then expressed in percentage. The disease incidence of guava seedling was determined by the formula. Percent disease incidence(PDI) of foliar diseases was determined by the formula. Percent disease severity (PDI) was determined by the formula. Meterological data collection: Meterologial data of the experimental period were collected from Meterological Department, Agargaon, Dhaka. Satistical analysis: Data on different parameters were analyzed in two factor randomized block design (RCBD) through computer software MSTAT-C. Duncan’s Multiple Range Test (DMRT) and Least Significant difference (LSD) test were performed for mean separation of different parameters and to determine the level of significant differences.

Source of Information: Int. J. Sustain. Crop Prod. 10(3):21-31, 2015

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study on the incidence and severity of seedling anthracnose disease of guava in different nurseries revealed that the diseases studied are related to the temperature, relative humidity and rainfall and have a profound effect on the disease. These weather parameters should be critically observed for each host-pathogen interaction and to find out the most appropriate time to combat the disease at minimum cost.

Knowledge Management: Journal