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Efficacy of Fungicidal Seed Treatment in Controlling Alternaria Spp. in Radish Seed

S. S. Islam
Postgraduate Student
Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur

M. H. Rahman
Scientific Officer
Hybrid Rice Project, Bangladesh Rice Research Institute (BRRI), Gazipur

M. J. Hasan
Senior Scientific Officer
Hybrid Rice Project, Bangladesh Rice Research Institute (BRRI), Gazipur

M. Asaduzzaman
A G M
Supreme Seed Co., Uttara, Dhaka

M. M. Khatun
Scientific Officer
OFRD, BARI, Rangpur, Bangladesh

Abstract/ Executive Summary:

The experiments were conducted to find out effective seed treating fungicides to control seed borne Alternaria spp. of the crop. In the experiment, seeds were treated with the six fungicides namely Rovral 50 WP (Iprodione) @ 0.20%, Dithane M-45 (Mancozeb) @ 0.45%, Ridomil MZ 68 WP (Metalaxil) @ 0.20%, Bavistin 70 WP (Carbendazim) @ 0.10%, Tilt (Propiconazol) @ 0.20% and Cupravit (Copper oxichloride) @ 0.70% were tested. Their effectiveness was measured by plating the treated and untreated seeds on blotter in petridishes. All six fungicides significantly reduced seed borne Alternaria spp. associated with radish seeds. Rovral 50 WP and Dithane M-45 were found most effective in reducing the seedborne infection of Alternaria spp. and increasing seed germination. Radish seeds harvested from fungicidal sprayed plot and naturally infected unsprayed plots. Collected seeds were treated with the six fungicides. Considering the results of the experiments, Rovral 50 WP (Iprodione) @ 0.20% was noted as the best fungicide against seed borne Alternaria spp.

Keywords: Fungicidal Treatment, Radish seed, Alternaria spp.

Location: Plant Pathology Laboratory, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur

Start Date: 00-00-2004

End Date: 00-00-2005

Program Area: Pest Management

Commodity: Radish

Objectives:

To find out effective seed treating fungicides to control seed borne Alternaria spp. of the crop.

Methodology:

An in vitro experiment was conducted at the Plant Pathology Laboratory, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during 2004-2005 radish growing season. To find out the efficacy of seed treatment with fungicides to control Alternaria spp. in radish seeds, six fungicides namely Bavistin 50 WP (Carbendazim), Dithane M - 45 (Mancozeb), Rovral - 50 WP (Iprodione), Ridomil MZ - 68WP, Cupravit (Copper oxichloride), and Tilt were tested. A seed sample of radish having natural infection with Alternaria spp. was treated with individual fungicides @ 0.25%. For treatment, 100 g of seeds and required amount of individual fungicide were taken in a conical flask. The flask was tightly closed with a polystyrene cork and shaken with hands for 10 minutes for uniform coating of seeds with the fungicide. To determine the effect of the fungicides on prevalence of Alternaria spp. and germination of the treated seeds were tested following blotter method. Treated seeds were placed on moist sterilized blotter paper in petridishes. Twenty-five seeds were plated on the blotter in each petridishes maintaining uniform distance. Seeds without fungicidal treatment were also plated, which served as control. The petridishes were incubated on the desk of Microbiology Laboratory at room temperature (25±4^oC). Associated Alternaria spp. was identified. Seeds harvested from different fungicides treated plots were also treated using respective fungicides which were used in field treatment. The prevalence of Alternaria spp. and germination were recorded following blotter method. Statistical analysis: All the collected data were analyzed for ANOVA using MSTAT-C computer program. Means were compared using DMRT to evaluate the differences among them. Relationships of seed yield/plot and germination of seeds with PDI of Alternaria blight (Alternaria spp.) and relationship of seed germination with percentage of seeds yielding Alternaria spp. were also determine following the same computer program.

Source of Information: Int. J. Sustain. Crop Prod. 2(5):46-50, 2007

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Incidence of seed borne Alternaria spp. and seed germination were recorded after 7 days of incubation at room temperature. All the fungicides significantly reduced the seed borne infection of Alternaria spp. Rovral 50 WP and Dithane M-45 were found most effective in reducing the seed borne infection of Alternaria spp. Significant increase in germination was observed in fungicide treated seeds over control. Rovral was found the most effective in increasing the rate of seed germination of radish.

Knowledge Management: Journal