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Biochemical Alteration of Cellular Components of Ash Gourd Due to Infection of Three Different Viruses

A. Muqit
Senior Scientific Officer
Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701

A. M. Aakanda
Professor
Dept. Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agriculture University, Salna, Gazipur-1706

K. A. Kader
Senior Scientific Officer
Plant Pathology Division, TCRC, BARI, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to determine the changes in ash gourd (Benincasa hispida) due to infection of Bottle gourd mosaic virus (BGMV), Watermelon mosaic virus 2 (WMV2) and Papaya rings pot virus (PRSV). Chlorophyll, β-carotene, organic carbon and DNA were found to be reduced in the infected leaves as compared to non infected ones. But total phosphorus and RNA contents were increased in the infected leaves. Ratio of chlorophyll a to b and RNA to DNA were higher in the virus infected leaves. Total nitrogen and protein was decreased due to BGMV and WMV2 infection while it was increased in case of PRSV.

Keywords: BGMV, WMV2, PRSV, Biochemical change

Location: The research farm of Bangabandhu Sheikh Mujibur Rahaman Agricultural University (BSMRAU)

Start Date:

End Date:

Program Area: Pest Management

Commodity: Ash gourd

Objectives:

To determine changes in concentration of chlorophyll, β-carotene, organic carbon, nitrogen, protein, phosphorus and nucleic acid in ash gourd due to infection of Bottle gourd mosaic virus (BgMV), Watermelon mosaic virus 2 (WMV2) and Papay ringspot virus (PRSV) under natural condition.

Methodology:

An ash gourd line “CQ-90-10” was grown during summer (May to August) at the research farm of Bangabandhu Sheikh Mujibur Rahaman Agricultural University (BSMRAU). The seeds of the ash gourd line were obtained from department of Genetics of Plant Breeding, BSMRAU. At flowering stage, leaf samples of apparently healthy and diseased plants were collected when typical symptoms of BgMV, WMV2 and PRSV infection was developed. Young leaves were collected from identical position of the main stem for laboratory test. Modified dot Immuno binding assay (DIBA) method was performed for the presence of virus. Chlorophyll and β-carotene contents were estimated respectively using double beam spectrophotometer (model 1200-20 Hitachi) extracting with 80% acetone. Total nitrogen and protein was determined by Kjeldahl method. Organic carbon was determined. Phosphorus and nucleic acid contents were estimated according to Hunter method and Spirin method respectively.

Source of Information: Int. J. Sustain. Crop Prod. 2(5):40-42, 2007

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In most of the cases, similar trend of reduction or increase in various cellular components due to virus infection were observed in the present investigation. But there is wide variation in the degree of change. This phenomenon suggests that extent of damage might not be the same by all the viruses. BGMV was found to reduce chlorophyll, β-carotene and organic carbon in greater degree than other viruses. So this virus might be more damaging in ash gourd than PRSV and WMV2. But this has to be confirmed by experimentation.

Knowledge Management: Journal