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Effect of Chemicals and Environment FRIendly Components on Growth Parameters and Yield Contributing Character of Onion (allium Cepa)

Md. Harun Or Rashid
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Md. Maksudul Haque
Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh

Dr. Md. Abu Bakr
Ex- Chief Scientific Officer
Division of Plant Pathology, Bangladesh Agricultural Research Institute, Gazipur 107, Bangladesh

Dr. Md. Rafiqul Islam
Professor
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Abstract/ Executive Summary:

Field experiment was conducted at spices Research Centre, BARI, Shibgonj, Bogra to determine the integrated approach for the management of purple blotch of onion for seed production. The treatment of the expeirment were Rovral wp @ 0.2% (T₁), Rovral wp @ 0.1% + Provax 0.25% (T₂); Trichoderma 5x106 spore/ml @ 100 ml/plant (T₃); Rovral wp @ 0.1% + Bavistin 0.5% (T₄); Neem leaf extract 1:6 (w/v) (T₅); Rovaral wp @ 0.1% +Evaral @ 0.1% (T₆); Rovaral wp @ 0.1% +Ridomil gold MZ-72 @ 0.1% (T₇); Rovaral wp @ 0.1% +Secure @ 0.05% (T₈) and control (T₉). Different treatment has effect on inhibition of mycelial growth of the fungus. The lowest mycelial growth was recorded in case of T₇ followed by T₂ where the highest mycelial growth was recorded in case of control (T₉). The highest plant height (81.40cm) and the yield (1.24 ton/ha) were recorded in treatment T₇ where as the lowest plant height (61.20 cm) and seed yield (0.82 ton/ha) were recorded in control (T₉).

Keywords: Chemicals,Environment, Isolation, Yield and onion (Allium cepa)

Location: Spices research centre, BARI, Shibgonj, Bogra

Start Date:

End Date:

Program Area: Pest Management

Commodity: Onion

Objectives:

To determine the integrated approach for the management of purple blotch of onion for seed production

Methodology:

The materials used and methodologies adopted for the research works are elaborately described in this chapter. It included a description of both in-vitro screening of fungicide and in-vivo efficacy of the fungicides and botanical extracts under field conditions. These comprised isolation of causal pathogens from infected onion plant and bioassay of fungicides, plant extract & bioagent against the test pathogen (Alternaria porri) under laboratory condition. The diseased leaves were cut into pieces (4 mm diameter) and surface sterilized with HgCl₂ (1:1000) for 30 seconds. Then the cut pieces were washed in water thrice and were placed on to acidified PDA in Petri dish. The plates containing leaf pieces were placed at room temperature for seven days. When the fungus grew well and sporulated, then slides were prepared and was observed under microscope and identified with the help of relevant literature. Altogether there were 09 different treatments as stated bellow. The treatments were applied into the assigned plots as per design of the experiment. T₁ = Rovral 0.2% (Recommended), T₂ = Rovral + Provax (0.1% + 0.25%), T₃ = Trichoderma (5x106 spore/ml), T₄ = Rovra l+ Bavistin (0.1% + 0.5%), T₅ = Neem leaf extract (1:6 w/v), T₆ = Rovral + Evaral (0.1% + 0.1%), T₇ = Rovral + Ridomil (0.1% + 0.1%), T₈ = Rovral + Secure (0.1% + 0.05%) and T₉ = Control. At recommended doses suspension/solution of fungicides were prepared by mixing thoroughly with requisite quantity of normal clean water. Spray was given at seven days interval in the assigned plots. The spray solution of fungicides used in the experiment were presented below with their doses. The diseased leaves were cut into pieces (4 mm diameter) and surface sterilized with HgCl₂ (1:1000) for 30 seconds. Then the cut pieces were washed in sterile water thrice and were dried keeping in untreated blotting paper then placed on to acidified PDA in petridish. The plates containing leaf pieces were placed at room temperature for seven days. When the fungus grew well, and sporulated, then the pathogen slide was prepared and was identified under microscope with the help of relevant literature. After incubation the mycelial growth of the fungus from each concentration of the fungicide was recorded. Data were analyzed statistically using MSTAT Computer Program. Data were transformed, whenever necessary, following Arcsine transformation. Means of treatment were separated using Duncan’s Multiple Range Test (DMRT).

Source of Information: J. Agric. Food. Tech., 5(2)8-14, (2015)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Field experiment with different fungicides alone and in combination of Neem extract (a botanical) and Trichoderma (a bioagent) was conducted. The performance of ecofriendly components Trichoiderma and Neem leaf extract against the disease were signicantly better than control but not up to the mark compared to the fungicide alone or in combination.

Knowledge Management: Journal