Md. Shawquat Ali Khan
Agronomy Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
M. Abdul Karim
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh
Abullah-Al-Mahmud
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Shahana Parveen
Irrigation and Water Management Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur, Bangladesh
Md. Mahfuz Bazzaz
Agronomy Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Md. Altaf Hossain
Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Salt Stress, Water Stress, Relative Water Content, Xylem Exudation and Proline Content
Vinyl house of the Department of Agronomy of Banghabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur
Crop-Soil-Water Management
The experiment was conducted in a vinyl house of the Department of Agronomy of Banghabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur, Bangladesh during winter, 2012. The location situated at about 240 23´ north latitude, 900 08´ east longitude and an altitude of 8.4m above sea level and adjacent to capital Dhaka city. Three tested genotypes of soybean (Glycine max L.) namely Galarsum, BD 2331 and BARI Soybean-6 were compared by six environmental conditions of salinity and water stress. Five sown seeds in the soil medium on January 20, 2012 in each plastic pots; 30 cm in height and 24 cm inner diameter. Each pot contained 12 kg air dried sandy loam soil. The soils of each pot were fertilized uniformly with 0.30 g of urea, 0.90 g of triple super phosphate, 0.60 g of muriate of potash and 0.60 g of gypsum. The pots were watered with the amount of 200 ml daily for easy germination. After the emergence and establishment, two uniform healthy seedlings per pot were allowed to grow for three weeks in equal environment. Protective measures were taken to control Jassids and white flies at vegetative stage. The environmental conditions were Control, Water shortage (irrigation with 70% depletion of available soil water when wilting sign developed), 50 mM NaCl irrigation, 50 mM NaCl irrigation + Water shortage, 75 mM NaCl irrigation, and 75 mM NaCl irrigation+ Water shortage. In salt water irrigation and water shortage treatments, initially all pots were irrigated with salt water for a week then followed water shortage, and thereafter salt water irrigations. The control plants were irrigated with tap water only with maintained field capacity. The experiment was arranged in Completely Randomized Design (CRD) with 3 replications. Different physiological parameters like Relative water content (RWC), Leaf water potential (LWP), Leaf temperature, Xylem exudation rate and proline accumulations were determined after 4 weeks of treatments imposition.
Following equation used to calculate relative water content of leaf:
Relative water content (RWC) = {(FW–DW)/(TW-DW)}X100
where, FW =Fresh weight, DW=Dry weight, TW=Total weight
Xylem exudation rate was calculated by deducting cotton weight from the sap plus cotton weight and expressed per hour basis as follows:
Exudation rate = {(Weight of cotton + sap) - (Weight of cotton)} / Time (h)
Leaf water potential was measured with Scholander Bomb Technique. Proline was determined by ninhydrin method. Leaf temperature was measured by a hand held Infrared thermometer. Data were analyzed by STAR (Statistical Tool for Agricultural Research) program and the treatments means were compared by using Tukeys’s Honest Significant Difference (HSD) Test. Differences at P ≤ 0.05 were considered significant.
Journal of Plant Sciences; 3(5): 272-278, (2015)
Journal