I. Ahmed
Lecturer,
Department of Agricultural Chemistry, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M.A.H. Chowdhury
Professor
Department of Agricultural Chemistry, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M.T. Islam
Professor
Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh
Arsenic, Seed germination, Root length, Shoot length, Rice varieties
Graduate laboratory of the department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur
Risk Management in Agriculture
The experiment was conducted in the graduate laboratory of the Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur (24.0000N and 90.4250E), Bangladesh, during July to August 2011. Nine varieties of rice such as BR 11 (Mukta), BR 22 (Kiron), BRRI dhan29, BRRI dhan33, BRRI dhan39, BRRI dhan40, BRRI dhan47, BRRI dhan49, and BRRI dhan53 were used as test crop and collected from Bangladesh Rice Research Institute (BRRI). Germination test of different varieties of rice seeds was conducted under different levels of As. Six (6) levels of As were used viz. 0, 15, 40, 70, 100, 150 mg L-1 As. Twenty-five seeds of each test variety were placed in petridish filled with tissue paper. Desired concentrations of As were calculated from sodium arsenite (NaAsO2). 10 mL of test solution was placed in each petridish. The tissue paper was kept moist constantly with distilled water. The experiment was arranged in a completely randomized design (CRD) with three replications at room temperature (28±1ºC). Data were recorded after seven days of sowing.
Seed germination: For each treatment, data were counted daily and sum of the data after 7 days were calculated using the formula given below:
Germination percentage = No. of seeds sown ÷ No. of seeds germinated × 100
Seedling shoot and root length: The shoot and root length of seedlings were measured after 7 days of the respective germination setting. Ten seedlings were carefully uprooted randomly out of all the seedlings grown from all petridishes of each treatment with the help of forceps. The excess water was soaked with tissue paper. Shoot and root portions were separated with the help of scissor. Shoot and root length were measured in cm.
Seedling shoot and root fresh weight: Ten shoot and root samples of the above samples were packed separately in paper bags and shoot and root fresh weights were recorded by an electronic balance. Seedlings' fresh weights were measured in gram (g).
Seedling shoot and root dry weight: After taking fresh weight those ten shoot and root sample packages were dried in an electric oven maintaining 72ºC temperatures for 48 hours. After drying, the shoot and root dry weights were weighed by an electronic balance and they were recorded accordingly. Seedlings' dry weights were also measured in gram (g).
Statistical analysis: All experimental data were subjected to analysis of variance (ANOVA) using statistical computer package MSTAT-C. Mean values were compared by the LSD or DMRT at the 5% level.
Int. J. Expt. Agric. 4(1):1-6 (2014)
Journal