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Rapid in Vitro Clonal Propagation of a Hybrid Muskmelon (cucumis Melo L.) Cultivar from Seedling Explants

Hafizur Rahman
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

M Shahinozzaman
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

M Rezaul Karim
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

M Aminul Hoque
Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi 6205, Bangladesh.

M Monzur Hossain
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

AKM Rafiul Islam
Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Abstract/ Executive Summary:

An investigation was undertaken to develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation. Best in vitro seed germination was observed in MS medium containing 0.5 mg/l GA₃. Two explants excised from axenic seedlings were tested for shoot proliferation and leaf node showed better shoot proliferation than shoot tip explants. Maximum shoots (8.22±0.23) per culture were produced from leaf node explants cultured on MS medium augmented with 1.0 mg/l BA. Rooting was found best on 0.1 mg/l NAA containing medium. Rooted plantlets were acclimatized gradually and transferred to the field condition and finally, 82 % plantlets were survived well.

Keywords: Muskmelon, Clonal propagation, Seedling explant, Leaf node

Location: Plant Breeding and Gene Engineering Laboratory, Rajshahi University, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Muskmelon

Objectives:

To develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation.

Methodology:

Hybrid seeds of muskmelon were collected from a Japanese seed company named TAKII Seed by Plant Breeding and Gene Engineering Laboratory, Rajshahi University, Bangladesh. Seeds were washed thoroughly under running tap water for 5 minutes and then washed with continuous agitation in a few drops savlon containing water for 10 minutes. Washed seeds were then treated with 0.1 % HgCl₂ for 8 minutes under laminar air flow cabinet to disinfect them. Finally, seeds were washed 3 to 5 times with sterile distilled water and were placed in conical flask (10-12 seeds per flask) containing MS medium alone or combined with GA₃ at 0.1-2.0 mg/l. After seed germination, two different types of explants viz. leaf node and shoot tip (1-1.5 cm in length) were excised and cultured on MS basal medium containing 3 % (w/v) sucrose and 0.8 % (w/v) agar with BA (6-benzyl adenine) and Kn (6-furfuryl amino purine) alone or in combination at different concentrations (0.5-2.0 mg/l) to test the effect of plant growth regulators on in vitro shoot multiplication. Well formed shoots were excised from in vitro derived shoot clumps and then cultured on MS medium supplemented with IAA (Indol-3 acetic acid), IBA (Indol-3 butyric acid) and NAA (α-napthalene acetic acid) alone at 0.1-2.0 mg/l for adventitious root induction. Finally, rooted plantlets were transferred to small pots containing garden soil and vermicompost (2:1) and before transferring them to the experimental field plantlets were maintained carefully for almost 21 days under laboratory condition and outdoor condition. During the investigation, the pH of the medium was adjusted 5.7±0.1 before autoclaving at 121 °C for 20 minutes at 1.2 kg/cm² pressure. All the cultures were maintained at 25 ºC±2 ºC (room temperature) under the cool white fluorescent lights for 16 hours photoperiod at 2000-3000 lux. Data on shoot proliferation were recorded after four weeks of culture and root induction data were recorded after three weeks of incubation.

Source of Information: Int. J. Agr. & Agri. R. Vol. 2, No. 1, p. 47-52, 2012, ISSN: 2223-7054

Article Link (Online Journal): http://www.innspub.net/wp-content/uploads/2012/02/IJAAR-V2No1-p47-52.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Maximum shoots ( 8.22±0.23) per culture were produced from leaf node explants cultured on MS medium augmented with 1.0 mg/l BA. Rooting was found best on 0.1 mg/l NAA containing medium. Rooted plantlets were acclimatized gradually and transferred to the field condition and finally, 82 % plantlets were survived well.

Knowledge Management: Journal