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Assessment of Stress-tolerance Attributes in Wheat Using Gene-specific Molecular Markers

Rona Mahmud
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh

M. A. Y. Akhond
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh

Abstract/ Executive Summary:

The experiment was under taken for Molecular characterization of rye chromosome translocations in twenty two local and two Australian wheat varieties. The effects on genetic background and environment on drought tolerance of lines potentially conferred by rye translocaton has been documented previously. Wheat-rye translocations, particularly those involving the short arm of rye chromosome 1 R (IRS). have been widely used in wheat breeding programmes worldwide. Amplification of the specific band was defected from 19 samples showing the presence of' rye translocation and 5 samples did not amplify the specific product for the rye translocation. These results encourage the development and use of the 1RS.1AL and 1RS.IDL translocations in wheat breeding programs. Further research is necessary for confirmation of the initial findings.

Keywords: Wheat, Rye Chromosome, Molecular markers

Location: Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Wheat

Objectives:

Objective of the study was to determine the present study at generating markers the aid breaking up the agronomically important wheat, and to eliminate segregates lacking any rye chromatin.

Methodology:

UNA was extracted from leaf tissue of 24 wheat varieties, using of Genomic DNA Mini Kit (plant) protocol and PCR analysis instructions for plant samples, Final concentrations of the reagents used in the Polymerase chain reaction (PCR) amplification was: 25 JlI reaction volume containing 2.5µI 10X buffer, 2.5 mM dNTPs, 5U/µl Taq DNA polymerase, 2.0 JµI each primer, 50 ng/µl DNA template. PCR conditions for the primer combination RlS F-RIS R, were as follows: Denaturing step: 94°C, 3 min. Amplification step: (35 cycles); 94°C in 30 see, 52°C in 1 sec, noc in 30sec. Extension step: 7 minutes at 72°C. PCR products were separated on 1.5% agarose gels and visualized after ethidium bromide staining using standard procedures.

Source of Information: Annual Research Report (2013-2014), Biotechnology Division, BARI, Gazipur

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Twenty four wheat lines/varieties were tested for the presence of rye chromosome segments using specific DNA markers. PCR analysis was carried out to identify a single product of 110 bp (all 1BL. 1RS and 1AL.1RS translocation lines). Amplification of the specific band was detected from the samples containing rye DNA but not from those lacking rye DNA (Driscoll and Sears 1971). RIS primers amplified specific bands from 19 samples showing the presence of rye translocation and the other hand 5 samples did not amplify the specific product for the rye translocation.

Knowledge Management: Report/Proceedings