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Seed Germination Potential, Phytochemical Analysis and Antioxidant Activity of Two Tomato Varieties

Mst. Samima Nasrin
Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Fatema Jesmin
Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Mostafizur Rahman
Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

M. Firoz Alam
Plant Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Abstract/ Executive Summary:

The present study was carried out to evaluate seed germination potential, phytochemical analysis and antioxidant activity of two tomato varieties viz., Raton and Persimmon. Seed germination experiment was undertaken with an objective to determine how the rate of seed germination can be influenced by various concentrations (10^-3 M, 10^-4 M, 10^-5 M) of plant hormones i.e. NAA and BA.Raton (BA 10^-5 M) showed highest germination percentage as well as the longest shoot and root length in contrast to other treatments. So, low concentration of plant hormones has sh owed more effectiveness for seed germination in both varieties. Phytochemical analysis and antioxidant activity of well ripen berry powder have assessed to explain the scientific basis for nutritional and nutraceuticals benefits of tomato. Phytochemical screening of Raton and Persimmon showed that alkaloids, flavonoids, tannins, terpenoids and glycosides are present in both Raton and Persimmon, but saponin and phenol are absent in Persimmon. Nutraceutical especially antioxidant activities of Raton and Persimmon have examined through DPPH free radical scavenging assay. Both the varieties showed significant antioxidant activity. Where IC₅₀ value of Raton and Persimmon are 29.2 mg/ml and 38.1 mg/ml respectively. The IC₅₀ value of standard sample (ascorbic acid) is 11.5 mg/ml. The result of this study indicates that Raton contain higher antioxidant potential than Persimmon.The phytochemical analysis supportsantioxidant properties of both varieties and also indicating for developing herbal medicine from tomatoes.

Keywords: Tomato, Seed germination, Phytochemical analysis, Antioxidant activity, DPPH, Free radicals

Location: Department of Botany, Rajshahi University, Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Tomato

Objectives:

To evaluate seed germination potential, phytochemical analysis and antioxidant activity of two tomato varieties viz., Raton and Persimmon.

Methodology:

Seed of Bangladeshi tomato variety “Raton” was collected from local farmer of Rajshahi-6205, Bangladesh. Seed of American tomato variety “Persimmon” was collected from Dr. Arun K Basak, Professor Emeritus, Department of physics, Rajshahi University, Bangladesh and was grown in home-garden. The identities of both varieties were confirmed by taxonomist Dr. A.H.M. Mahbubur Rahman, Associate Professor, Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh.6-benzylaminopurine (BA), α-napthaleneacetic acid (NAA), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ascorbic acid, methanol, ethanol, Dragendroff’s,Hager’s, Mayer’s, Wagner’s and Tannic acid reagents. Seeds were treated for 10 min in 70 % alcohol, then washed and rinsed with distilled water. These surface sterilized seeds were soaked in solution of (10^-3 M, 10^-4 M and 10^-5 M) NAA and BA and maintained in a growth chamber in darkness for 24 h at 25 °C. Seeds, soaked in distilled water were considered as control. For each variety, 15 seeds with three replicates per treatment were sown in a Petri dish 9 cm in diameter, containing a single layer of sterile blotting paper, and germinated in an incubator at 28 °C. Then, dishes were moistened with equal amounts of 5 ml consecutively distilled water in control and various hormonal solutions as treatments. Observation aspects like germination count (recorded for 5 days), measurement of root and shoot length was measured (recorded for 7 days). First, various concentrations like 20, 40, 60, 80, 100 mg/ml of sample in methanol were prepared. 2 ml of methanol solution of plant sample or standard at different concentration was taken in test tube. 3 ml of 0.1 mMmethanol solution of DPPH was added into the test tube. The test tube was incubated at room temperature for 30 minutes in dark place to complete the reaction. Then the absorbance of the solution was measured at 517 nm using a spectrophotometer against control. Ascorbic acid was used as standards (positive control). Then the percentage (%) inhibition activity was calculated. % I={(A_O– A₁)/A_O} * 100. Where,A_O is the absorbance of the control, and A₁ is the absorbance of the sample or standard. Sample was analyzed in two replications and data presented as mean (±) standard Error (SE). Then % of inhibition was plotted against blank concentration and from the graph IC₅₀ was calculated. IC₅₀ value, the concentration of sample required for 50% scavenging of DPPH free radical are completed. Statistical analysis was conducted using analysis of variance(ANOVA). Least Significant Difference (LSD) test was used to speculate further if there was a significant difference within varieties, various concentrations and plant hormones. P values <0.05 were considered as significant.

Source of Information: International Journal of Biosciences, Vol. 8, No. 1, p. 63-762016, ISSN: 2220-6655

Article Link (Online Journal): http://www.innspub.net/wp-content/uploads/2016/02/IJB-V8No1-p63-76.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the above discussion, it can be concluded that as tomato is easy to seed germinate and because it is ranked among the most important crops throughout the world, the improved capacity of seed germination by the plant hormones could be useful in increasing commercial exploitation of tomato in the field of biotechnology. Moreover, qualitative phytochemical screening and nutraceutical like antioxidant activity of tomato suggests that tomato has a rich amount of valuable ingredients that are beneficial for health. So, further investigations on the isolation and identification of bioactive components would help to ascertain its potency.

Knowledge Management: Journal