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Validation Trial of Tissue Cultured Plantlets of Jackfruit

M. M. H. Molla
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

P. K. Sinha
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. Z. Rahman
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. A. Rouf
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Z. A. Firoz
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. R. Dhar
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

R. U. Shamim
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. M. Khatun
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

D. Khanarn
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

A. Y. Akhond
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Tissue cultured, seeded and grafted saplings of Jackfruit were planted to observe the field performance on growth and yield. Highest plant height was found in Tissue cultured plants (414.2 cm) followed by seeded (336 cm) and grafted (285.83 cm). Seeded BARl kathal-2 plants produced 284.5 cm plant height. Highest base girth (18.1 cm) was also recorded in tissue cultured plants followed by grafted (14.33 cm) BARl kathal-2. Also, highest number of branch/plant was recorded in tissue cultured plants followed by grafted where minimum was in seeded plant. Maximum plant canopies were recorded also in tissue cultured plants followed by grafted and seeded plants both in North-South & East- West direction. There was no male or female flowering had been seen any of tissue cultured, grafted and seeded plants up to third year.

Keywords: Tissue culture, Jackfruit, Grafted and Seeded

Location: Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Jackfruit

Objectives:

Objective of this study was to see the field performance of tissue culture saplings of Jackfruit compared to grafted and seeded ones.

Methodology:

The regenerated rooted plantlets with 5-6 leaf stage were transferred from culture room and kept in room temperature (30°C) for 5-7 days. The plantlets were then removed from the culture tubes and all the adhering media were carefully washed out so that, the root damage was the least. Washed plantlets were planted into small polyethylene begs containing sterile soil, sand and decomposed cow dung at the ratio of 1: 1: 1. Subsequently, the healthy plants were transplanted in a 9 x 12 inch earthen pots containing soil mixture at nursery for three months. Well established fifteen saplings were transplanted along with grafted and seeded ones at Biotech Field, Gazipur; Hill Agricultural Research Station, Khagrachari Hill Districts; Hill Agricultural Research Station, Ramgarh, Khagrachari Hill Districts; Regional Agricultural Research Stations, Hathazari, Chitagong & Akbarpur, Sylhet and Oodaleah Tea Estate, Nagirhat, Chittagong on May, 2011. Saplings were transplanted at single line design following plant to plant distance 10.0 m. Single plant was considered as one replication. Data on Plant height, base girth and number of branches/plant and plant canopy were recorded.

Source of Information: Annual Research Report, Biotechnology Division, BARI, 2013-2014

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Maximum plant canopies both North-South & East-West was recorded also in tissue cultured plants followed by grafted and seeded plants both in North-South & East-West direction. There was no male or female flowering was seen any of tissue cultured, grafted and 'seeded plants up to third year.

Knowledge Management: Report/Proceedings