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In Vitro Multiplication of Transgenic Katahdin Sp 951 and Non-transgenic Katahdin Potato Parent Materials

M. Mahmuda Khatun
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Objective of the study was to determine in vitro multiplication of transgenic katahdin SP 951 and non-transgenic katahdin potato parent materials. Transgenic Katahdin SP951 and non-transgenic Katahdin were used as parent materials. Sprouts of these varieties were used as explant. Sprouts were cultured on PROP medium and multiplied in vitro. From in vitro culture and green house production, a total of 604 numbers of transgenic Katahdin SP951 mini tubers and 543 numbers of non-transgenic Katahdin minitubers were harvested. Produced minitubers were stored in Debigonj, BARI cold storage for planting in next season.

Keywords: Transgenic Katahdin SP951, Potato, Non-transgenic Katakin

Location: Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

Objective of this study was to determine in vitro multiplication of transgenic katahdin SP 951 and non-transgenic katahdin potato parent materials

Methodology:

Sprouts of transgenic Katahdin SP 951 and non-transgenic Katahdin were used as explant source. For inoculation of explants firstly potato sprouts were surface sterilized under running tap water for 30 min. with jet and trix to remove the surface contamination outside the clean bench. After that the sprouts were taken inside the laminar air flow cabinet in a autoclaved jar and dipped in 99% ethanol plus 2 drops of Tween-20 for 20 sec. and washed with autoclaved distilled water 2 to 3 times. Finally, sprouts were sterilized with clorox solution (60 ml clorox: 40 ml distilled water) for 1 min. and then washed for 3 to 4 times with autoclaved distilled water. After that the sterilized sprouts were excised with the help of the sterilized scalpel and forceps. Then the prepared explants were placed in a test tubes/baby jars containing previously prepared media. The inoculated cultures were placed in growth room at 25 ± 1⁰C under approx. 2000 lux by cool white fluorescent tubes for 16/8 light/dark cycle.

Source of Information: Annual Research Report, Biotechnology Division, BARI, 2013-2014

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Initiation of explant occurred within four weeks of culture. Shoots were produced straight way. Then single nodes were cut and put in the same medium for multiplication of these materials. After three to four subcultures plantlets were multiplied in vitro and a good number of plantlets were produced. The in vitro platelets were taken out from the growth room and acclimatized to greenhouse condition. The plantlets were successfully established in potting soil and growing well. After three months the minitubers were harvested and stored for next year trail.

Knowledge Management: Report/Proceedings