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Impact of Carbon Sources on Callus Induction and Regeneration Ability in Banana Cv. Sabri

Md. Jamal Hossain
Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Md. Abdul Bari
Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Nure Anzuman Ara
Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Md. Al Amin
Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Motiur Rahman
Dept. of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

Kazi Md. Faisal Haque
Dept. of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

The purpose of this study was to investigate the effect of three different carbon sources; sucrose, glucose and sorbitol alone and in their combinations on in vitro callus induction and regeneration in banana cv. Sabri. Male flowers were used as experimental material in this investigation. The male flowers were cut into small pieces and cultured on MS medium supplemented with 2 mg/l 2, 4-dichloro phenoxy acetic acid (2, 4-D) + 1 mg/l ?- naphthalene acetic acid (NAA) + 1 mg/l Indole-3-acetic acid (IAA) + 1 mg/l biotin+1 mg/l glutamine containing different percentages of sugars like sucrose, glucose and sorbitol singly and in their combinations. Glucose showed the highest performance rate for callus induction and 3 % concentration proved the optimal dose. Sucrose and sorbitol behave different in embryo formation and they produced the highest and lowest number of embryos, respectively in regeneration medium. In respect of overall performance the highest percentages shoot and root formation was obtained in the media containing 3% and 2% sucrose, respectively. These findings would be beneficial for further experiment of other cultivars of banana.

Keywords: Carbon sources, Sabri, Callus, Embryogenesis, Regeneration

Location: Biotechnology Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Banana

Objectives:

To study on the effect of carbon sources on callus induction and regeneration in banana.

Methodology:

In the investigation, male flowers of Musa spp. cv. Sabri were used as experimental materials. The initial plant material consisting of male inflorescences of banana were collected from field grown plants. Male flowers were collected from 10th to 18th hands of the inflorescences after 6-10 weeks of flower opening. Under a sterile condition, the outer overlapping bracts were removed and the remaining part (2-3cm in length) containing male flowers were isolated and surface sterilized by 70% ethyl alcohol for 1 minute. The male flowers were cut into small pieces and they were transferred in petri dishes (9 cm in diameter) containing MS medium which was supplemented with 2 mgl^-12,4-D +1mgl^-1NAA+1mgl^-1 IAA +1mgl^-1 Biotin+1mgl^-1glutamine and different percentages (0%, 1%, 2%, 3%, 4%) (w/v) of different sugars: sucrose, glucose, sorbitol and their combinations which were autoclaved in 121°C temperature for 20 minutes and agarifided with 8 gml^-1 agar. The pH of the medium was adjusted to 5.8. The media were autoclaved and plant growth regulators were sterilized using microbiological filter (pore size 0.2 μm). The explants (10 per petridish) were cultured in the continuous dark at 25±2 °C. For embryo formation, the calli derived from male flowers, were subcultured on the agarifide MS medium supplemented with different carbon sources. After 80-110 days the embryos were appeared in the solid MS medium. The embryos showed various polarities like unipolar and bipolar. The number of embryos per callus was calculated under microscope from the beginning of embryogenesis. For shoot and root formation, the bipolar embryos were transferred in MS medium containing different carbon sources and 0.5mgl-1BAP+2mgl^-1IAA. The data of the formation of callus, roots and shoots were taken and the results of these experiments are expressed as either mean value or a percentage.

Source of Information: International Journal of Biosciences | IJB |; ISSN: 2220-6655 (Print) 2222-5234 (Online); Vol. 3, No. 1, p. 156-163, 2013

Article Link (Online Journal): http://www.innspub.net

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Among the different carbon sources used, glucose performed well followed by sucrose and sorbitol in terms of callus induction. Although sucrose and sorbitol are better carbohydrate choices for embryo formation in callus regeneration of banana. To ascertain,, further research would be required to explore in vitro plant regeneration of other cultivars of banana.

Knowledge Management: Journal