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Sero-prevalence of Mycoplasma Gallisepticum Infection of Chickens in Model Breeder Poultry Farms of Bangladesh

S. K. Sarkar
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

M. B. Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

M. Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

K. M. R. Amin
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

M. F. R. Khan
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

M. M. Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2200, Bangladesh.

Abstract/ Executive Summary:

The sero-prevalence of Mycoplasma gallisepticum (MG) infection of chickens in selected Model Breeder Poultry Farms was determined during the period January to May, 2004. To conduct this study a total of 382 sera samples were collected. Rapid Serum Plate Agglutination (SPA) test was performed using commercial MG antigen (Nobilis® MG) to detect the presence of antibodies against MG. The over all sera-prevalence of MG infection was 58.90% in the study area. The highest prevalence (62.44 %) of MG infection was found in winter season followed by summer season (53.10%). The result further revealed that the infection was higher (59.94%) in female birds than in male birds (48.57%). It was also demonstrated that the infection was higher (62.80%) in Feni sadar than in Chhagoalnaiya thana (53.45%).

Keywords: Sero-prevalence, Chickens, Model-breeder poultry farms

Location: Feni district

Start Date: 00-01-2004

End Date: 00-05-2004

Program Area: Animal Health and Management

Commodity: Diseases, Chicken

Objectives:

To determine the sero-prevalence of MG infection in chickens of selected Model Breeder Poultry Farms (MBPF) of Bangladesh.

Methodology:

The present study was conducted during the period from January to May 2004, at two Thana of Feni district, namely Feni Sadar and Chhagoalnaiya, where Smallholder Livestock Development Project-2 (SLDP-2) has been implemented by several NGO's. A Model breeder poultry farm consists of 60-66 birds in which Fayoumi hens and RIR cock were reared. Blood samples were obtained twice from each farm during winter (January-February) and summer (April- May) season. Standard Mycoplasma gallisepticum (Nobilis® MG) antigen manufactured by Intervet International, Holland was used for Rapid Serum Plate Agglutination (SPA) test for the detection of M. gallisepticum antibodies in the collected sera to determine the infection. A total of 382 blood samples were obtained aseptically from the wing vein of the selected birds using 5ml sterile disposable syringe and needles. The blood allowed to clot in the syringe and kept for 1-2 hours at room temperature. After clotting, sera were separated, centrifuged and poured in sterile vials, labeled individually and stored at 4°C until used. The sera so collected were transferred to the Laboratory, Department of Microbiology and Hygiene, BAU, Mymensingh, in ice pack condition in thermo flask for further testing. The SPA test was conducted with crystal violet stained Mycoplasma gallisepticum antigen (NobiliS® MG antigen). For this test 0.02 ml of antigen and 0.02 ml of chicken sera were placed side by side with a micropipette on a glass plate and mixed properly by stirring with small tooth pick followed by gentle rocking. Results were read within 2 minutes. In positive cases granules formed slowly which was seen during rocking, but in negative case no such granules formed within two minutes. All SPA test results were recorded.

Source of Information: International Journal of Poultry Science Volume 4, Number 1, 32-35, 2005

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Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The overall prevalence of MG infection was higher than Pradhan and Dulali which might be due to the maintenance of breeder stock for a long period of time and replacement of breeding stock with the progeny of the same flock. However, intensive nature of poultry farming provide opportunity for recycling of the pathogens due to population density. The other factors that contribute to MG infection are poor ventilation, infection of litters and no restriction on movement of technical personnel, visitors and such other persons as well as other bio-security measures.

Knowledge Management: Journal