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Establishment of an Efficient Method for Micropropagation of an Important Medicinal Herb (scoparia Dulcis L.) from Shoot Tips and Nodal Segments

M. H. Rashid
Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205

M. Khalequzzaman
Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi-6205

M. F. Hasan
Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi-6205

R. Das
Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi-6205

M. S. Hossain
Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi-6205

S. Mahbbat-E- Khoda
Research officer
Nutritional Biochemistry Lab, Laboratories Sciences Division, ICDDRB

Abstract/ Executive Summary:

The present experiment was conducted in the Laboratory of Biotechnology, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, during January to August 2008 to establish a standard protocol for plant regeneration as well as acclimatization of plantlets. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations of cytokinins for multiple shoot induction. The highest number of shoots (85.3) per explant was recorded on the MS medium supplemented with 1.0 mg/l BAP from nodal segment after 8 weeks of culture. The highest number of roots (21.5) per shoot was observed in the MS medium having 0.5 mg/l NAA. The well rooted plantlets were acclimatized and successfully transferred to natural condition, where 80% plantlets were survived.

Keywords: Micropropagation, Shoot tips, Nodal segments

Location: The Laboratory of Biotechnology, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205

Start Date: 00-01-2008

End Date: 00-08-2008

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

Objective of the study was to establish a standard protocol for plant regeneration as well as acclimatization of plantlets.

Methodology:

Shoot tips and nodal segments of Scoparia dulcis L. were collected from Rajshahi University Campus, Bangladesh. The explants were washed first under running tap water for 20 minutes and treated with 1% Tween 80 for 8 minutes followed by repeated rinsing with sterile distilled water. Further sterilization was done under aseptic conditions in a Laminar Airflow Hood. Explants were surface sterilized with 0.1% (W/N) HgCl₂ for 5 minutes. Finally, the explants were washed thoroughly with autoclaved distilled water for several times to remove the traces of sterilant. The explants were cut into appropriate size and cultured on MS medium. Throughout the experiments full strength MS medium with 3% (W/V) sucrose and gelled with 0.8% (W/V) agar was used. The pH of all media was adjusted to 5.8 prior to autoclaving. The cultures were incubated in a culture room at 25 ± 2°C with a photoperiod of 16 hour at 3000 lux light intensity provided by cool white fluorescent tubes. The basal medium was supplemented with different concentrations of cytokinins. Rooting of shoots was achieved on MS medium supplemented with different concentrations of NAA or IBA. Well rooted plantlets were removed from the culture vessels, washed gently under running tap water and planted in plastic pots containing a potting mixture of sand, soil and humus in the ratio of 1:2:2. The potted plantlets were covered by polythene sheet to maintain suitable humidity. After sufficient acclimatization, the plantlets were transplanted into the pot containing soil, where 80% plantlets were survived.

Source of Information: Int. J. Sustain. Crop Prod. 4(1):5-9, 2009

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present investigation, a suitable protocol was established through multiple shoot induction from nodal segment and shoot tip explants of Scoparia dulcis. This protocol can be exploited for commercial propagation and conservation of other valuable medicinal plant resources.

Knowledge Management: Journal