M. Hasanuzzaman
Associate Professor
Department of Genetics and Plant Breeding, HSTU, Dinajpur, Bangladesh
Faruq Golam
Institute of Biological Sciences, University of Malaya, Malaysia
Parent selection, Multivariate analysis, Dendogram, Chilli
Research and Development Farm of Lal Teer Seed Limited, Gazipur
Variety and Species
Materials: Twenty chilli genotypes were taken for preliminary evaluation and selection for detailed genetically as well as others studies on yield and yield related characters. Based on the degree of divergence among geontypes, the 20 accessions, CCA 1, CCA 2, CCA 3, CCA 4, CCA 5, CCA 6, CCA 7, BARI Morich 1, CCA 9, CCA 10, CCA 11, CCA 12, CCA 13, CCA 14, CCA 15, CCA 16, CCA 17, CCA 18, CCA 19, CCA 20 could be grouped into six clusters. These genotypes, selfed for several generations, were supplied by Lal Teer Seed Limited (Formerly East West Seed (Bangladesh) Limited). Method: The twenty different genotypes were grown in Research and Development Farm of Lal Teer Seed Limited, Basan, Gazipur during 2006-2007 starting from October, 2006. The experiment was laid out in randomized complete block design with three replications. The seeds of the 20 different chilli germplasm were sown in Seedling Tray. The media in the seedling tray have been prepared by using coconut coir, ash and decomposed cow dung at a ratio of 50%, 25% and 25% respectively. The media were boiled by steam for two hours. After cooling the media, the seeds were sown on 17 October, 2006. The seedlings at the age of 4 to 5 leaves were suitable for transplanting and this took 35-45 days after sowing. The transplantation of seedlings was done on December 5, 2006. Raised beds were prepared for transplanting. The width of raised bed was 1.5 meter. Plant to Plant distance was 50 cm and row to row distance was 70 cm. Bed to bed distance is 1.0 meter that was used as drain. Cow dung, Urea, TSP, MP, Gypsum and Zinc Oxide were applied @ 15 tons, 200 kg, 300 kg, 200 kg, 110 kg and 5 Kg per hectare respectively. The entire amount of cow dung, TSP, Zinc Oxide, Gypsum and one-third of the urea and MP is applied at the time of final land preparation while the rest of the urea and MP is applied at two equal installments, 25 and 50 days after transplanting. At the time of transplanting, Dursbarn 20 EC and Ridomil MZ 68 WP were used at the rate of 5 ml/L and 3g/L respectively for soil treatment. Irrigation was given as and when necessary. Weeding was done after every 20 days of transplanting. The diversity between accessions was assessed by multivariate statistics. Non-hierarchical pre determined cluster analysis was done using Genstat 5 Release 4.1 (Fourth Edition). The Mahalanobis distance (D2) was applied to measure the genetic divergence between the accessions. A canonical variate analysis was carried out to confirm the D2 analysis as well as to provide a graphic representation of the divergence of the accessions. A similarity dendrogram was plotted using the Minitab 15.1.0.0.
Int. J. Sustain. Crop Prod. 6(1):63-75, 2011
Journal