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In Vitro Seeds Germination and Plantlets Development of Silvius Appendiculatus (orchidaceae)

M. N. Hasan
Lecturer
Department of Genetic Engineering and Biotechnology, Jessore Science and Technology University, Jessore

A. Ansari
Lecturer
Department of Genetic Engineering and Biotechnology, Jessore Science and Technology University, Jessore

M. N. K. Azam
Biotechnology and Genetic Engineering Department, University of Development Alternative, Dhanmondi, Dhaka

S. M. A. Awal
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna – 9208, Bangladesh

Abstract/ Executive Summary:

Bangladesh is rich in orchids, with 159 species and two varieties under 63 genera. These species are distributed mainly in the hilly areas of greater Sylhet, Chittagong and Mymensingh districts. Seeds of S. appendiculatus were germinated successfully and grew well in the in vitro culture. The best medium were observed for germinating the S. appendiculatus seeds, viz. Knudson-C (KnC) basal medium supplemented with 30% (v/v) maturity coconut water/milk (CW). All the seeds were observed those overall germination, protocorms formation and seedling development (about 100%). The best medium for growing and development of seedlings to become fully expanded plantlets was determined half strength of (1/2 MS) medium supplemented with 40% (v/v) CW. The plantlets of S. appendiculatus were resulted from these research approximately more than 2000 ndividuals. The fully expanded seedlings were transferred to perforated plastic pots and grow in the screen house.

Keywords: Orchidaceae, Silvius appendiculatus, In vitro, Seed germination, Coconut water

Location: Plant Tissue Culture laboratory of the Department of Genetic Engineering and Biotechnology, JSTU, Jessore

Start Date: 01-02-2011

End Date: 01-09-2011

Program Area: Crop-Soil-Water Management

Commodity: Orchid

Objectives:

To clarify the effective concentration of growth regulators for in vitro seed germination and plantlets development of orchid.

Methodology:

The experiment was conducted from February 01 to September 01, 2011 at Plant Tissue Culture laboratory of the Department of Genetic Engineering and Biotechnology, Jesssore science and Technology University, Jessore, Bangladesh to clarify the effective concentration of growth regulators for in vitro seed germination and plantlets development of orchid. Preparation of explants: The green pods of S. appendiculatus were harvested from Chittagong district of Bangladesh for using as plant materials and propagated through in vitro seed germination. The dry petals attached the pods were removed, dipped the pods quickly into 95% alcohol and flame over a spirit lamp then transferred into a sterile Petri dish. The pods were cut across into 2 halves by using surgical blade. Before inoculating the seeds, the sizes of the seeds were measured by using a binocular microscope. Seeds germination: The seeds were spreads on the surface of KnC solidified medium supplemented with various concentration of mature coconut water (CW), viz. 0, 10, 20, 30, and 40% as treatments. The pH of all the media were adjusted to 5.2 before pour into 200 ml glass vessels. The glass vessels were filled in 25 ml medium then sterilized of the medium with 120^oC temperature and 1.2 cm2/kg pressures for 25 minutes by using autoclave apparatus. After the seeds inoculation on the surface of solidified medium, the culture vessels were incubated in the culture room with 25^oC temperature and 12 h photoperiod illumination by using tube lamp. The effect of treatments toward seeds germination was weekly recorded. The duration of seeds germination process was terminated when the protocorm-like bodies was formed. Seedling development: The basal mediums used for seedling development were KnC and 1/2MS. Both KnC and 1/2MS mediums were supplemented with 0, 10, 20, 30, and 40% CW as treatments. The protocorms with their shoot primordial were inoculated on the surface of both KnC and ½ MS medium. The culture vessels were maintained in the same culture room of the seeds germination point. The growth responses of seedling were also weakly observed. The experiments were terminated when the seedlings were fully developed (belonging roots, 3-4 leaves, and 4-5 cm high) and ready for acclimatization.

Source of Information: Int. J. Sustain. Crop Prod. 6(2):1-5, 2011

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Propagation of S. appendiculatus by in vitro seeds germination was successfully achieved. The best medium for germination of S. appendiculatus was KnC medium supplemented with 30% CW and the best medium for seedling development was 1/2MS medium supplemented with 40% CW. The plantlets of S. appendiculatus were resulted from these research approximately more than 1500 individuals. The fully expanded seedlings were successfully transferred to perforated plastic pots.

Knowledge Management: Journal