M. Aktaruzzaman
Department of Plant Pathology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh
D. B. Ray
Department of Plant Pathology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh
M. F. Hossain
Department of Plant Pathology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh
T. Afroz
Department of Plant Pathology, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh
BAU-Biofungicide, Pant extracts, Root-knot, Meloidogyne javanica, Papaya
Plant Pathology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur
Pest Management
Experimental site: The experiments were conducted at the Hajee Mohammad Danesh Science and Technology University, Dinajpur during December 2009 to May, 2010. The efficacy of BAU-Biofungicide, Bashak leaf extract, Pineapple and Mustard oil-cake extracts against root-knot of papaya was evaluated in individual set of pot experiment in the roof. Preparation of pot soil and potting: At first, sandy loam soil, sand and well decomposed cow dung were collected and mixed properly at the ratio of 2:2:1. Sandy loam soil, sand and well decomposed cow dung were collected from the field of HSTU campus; riverside of Punorvoba and dairy farm of HSTU, respectively. Then the mixed soil was sterilized with formalin at the rate of 3% per cubic feet soil. The treated soil was covered by brown paper for 72 hours without disturbance. After 72 hours, the brown paper was removed and the sterilized soil was exposed to air-drying for 48 hours in order to remove excess vapor of formalin. Collection and surface sterilization of seeds: Apparently, mature, healthy and disease free seeds of papaya var. BARI Papaya 1 were collected from BADC, Chahelgaji, Dinajpur. Surface sterilization of these seeds was done with low concentration of mercuric chloride solution (0.001%) for 1 minute and subsequently washing was done with water for three times. Collection of BAU-Biofungicide: BAU-Biofungicides were collected from Disease resistance Laboratory of Prof. Dr. Ismail Hossain, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh. Preparation of leaf extract: Bashak leaf was collected from the HSTU campus. Leaf (20g) was macerated in an electric blender and soaked separately in l00ml distilled water in several conical flasks and then it was filtered. Thus, these filtrates treated as standard Bashak leaf extract(S). The same procedure was followed for the preparation of leaf extract of Pineapple and Mustard oil-cake (MOC). Sowing of seeds on the pot: The treated seeds of papaya were sown in each pot (30 cm diameter) with sterilized soil. Optimum moisture in each pot was maintained through sprinkler irrigation, whenever it is necessary. Design of experiment: The experimental set was carried out to evaluate the effect of soil drenching with BAU-Biofungicide and plant extracts on the growth of 60 days and 90 days old seedling of papaya inoculated at 30 days of growth stage with Meloidogyne javanica after 7 days of drenching. All the pots were arranged in completely randomized design. Standard leaf extracts of Bashak (Adhatoda vasica), Pineapple (Ananas sp.) and Mustard oil-cake extracts (Brassica campestris) as well as BAU-Biofungicide were used per treatments. Altogether, 5(five) treatments including control were maintained in the experiment. Each treatment was replicated 3 times.Preparation of inoculum and inoculation: Egg-mass was collected from the roots of brinjal plant as previously inoculated individually with a single eggmass of Meloidogyne javanica. For inoculation, ten reddish brown mature eggmasses were placed in each pot around the standing plant in 2 holes (2.5cm deep), five eggmasses on each side of the plant. Inoculation was done on whole sets of pot plants at 37 days old seedling. Application of plant extract and BAU-Biofungicide solution: BAU-fungicide, standard leaf extracts of Bashak, Pineapple and Mustard oil-cake extracts were used in the following treatment:T0 = Control (untreated), T1 = BAU-Biofungicide (2%), T2 = Bashak leaf extract (S), T3 = Pineapple extract (S), T4 = Mustard oil-cake extract (S), Parameters studied After 30, 60 and 90 days of inoculation, plants were uprooted carefully to study the following characters: Length of shoot (cm), Length of root (cm), Weight of shoot g), Weight of root (g), Number of galls/g of root, Counting of galls, After washing, the roots were preserved in 5% formalin solution. The roots were cut into small pieces of (1cm) and one gram of root was randomly taken from the bulk to count the number of galls formed. Statistical analysis: Data on the length of shoot and root, fresh weight of shoot and root, number of galls/g of fresh root were analyzed statistically to find out the level of significance. The means for all the treatments were counted and the analysis of variance was studied by F-test for the treatment means and replication means. The mean differences were evaluated at P = 0.05 level by Duncan’s New Multiple Range Test. Correlation co-efficient and linear regression equations were determined.
Int. J. Sustain. Crop Prod. 7(1):1-5 (2012)
Journal