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Genetic Diversity of Morphological Traits of Brassica Napus Parents and F7 Family Lines

M. A. Awal
Horticulture Centre, DAE, Mehedibag, Sylhet, Bangladesh

M. K. Uddin
Department of Agrobiology, Niigata University, Japan

SK. N. Uddin
Department of Soil Science, BAU, Mymensingh, Bangladesh

S. E. Jahan
BADC, Sylhet, Bangladesh

L. Rahman
Department of Genetics and Plant Breeding, BAU, Mymensingh, Bangladesh

Abstract/ Executive Summary:

The research was conducted to test the performance of twelve selected F7 family lines evolved from the cross products of two Brassica napus varieties in comparing with their parents affiliated to some yield contributing characters. The characters, days to 50% germination, number of primary branches per plant, days to 50% flowering, average pollen fertility (%), plant height at maturity, number of siliqua per plant, beak length, number of seeds per siliqua, seed yield per plant were studied. There were significant variations among the varieties for all the characters except pollen fertility. But highly significant variations were found among the family lines and their parents in respect to morphological characters. The insignificant variation among genotypes tested for some traits denoted that, although the cross products are from two different parents, but have possibly been reached good balance due to high selection pressure in previous generations for types having better combinations. The family line 459 belonging to ED (BINASarisha-5 × BINASarisha-4) and family line 111 belonging to DE (BINASarisha-4 × BINASarisha-5) were found to be better in performance and have unveiled near close trails with parents in respect to the aforementioned traits. In general, genotypic correlation coefficients were higher than their corresponding phenotypic ones. Grain yield per plant had significant positive correlation with days to 50% flowering, average plant height at maturity, average number of siliqua per plant. Path coefficient analysis suggested that number of seeds per siliqua contributed maximum to seed yield through positive direct effect followed by average plant height.

Keywords: Brassica napus, F7 lines, Genetic diversity, Morphological traits

Location: Experimental Farm of Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh

Start Date: 30-06-2008

End Date: 30-11-2009

Program Area: Variety and Species

Commodity: Mustard

Objectives:

To find out the genetic variation in morphological traits of the progeny lines and their parents, the correlation co-efficient, the path co-efficient pattern of different characters and the better genotype based on performance of individual character as well as characters together.

Methodology:

Experimental site and duration: The study has been conducted as the partial fulfillment of MS degree at the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh during July, 2008 to December, 2009 and the Experimental Farm of the same department that located at 24^o75' N latitude and 90^o50' E longitude at an elevation of 18m above the mean sea level has been used for the experiment. Soil and climate: The experimental area was characterized by non-calcareous dark grey floodplain soil belonging to the Sonatola Soil Series under the Old Brahmaputra Floodplain (Agro-Ecological Zone 9). Soil of the experimental field was slightly acidic with a pH value of 6.17, low in organic matter and fertility level. The land type was medium high with silty loam texture and the climate of the place was characterized by wet summer and dry winter. Materials used: For the present study, two Brassica napus parents and 12 F7 lines were used in the experiment. The source and characteristics of the parental varieties and the progeny lines were described. Experimental design and layout: Field layout was done after final land preparation. The experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. The unit plot size was 3m x 1.5m with 30 cm distances between line and 50 cm distances between plots. Number of rows in each plot was 5. The distance between rows was 50 cm and between plants 5cm for proper development and growth of plants. Sowing of seeds: Selected parental materials and seeds of 12 family lines of Brassica napus were sown in lines in the experimental plots. The Seeds were placed at about 1.5cm deep in the soil. Intercultural operations: Intercultural operations such as weeding, thinning, irrigation and top dressing were done in all the plots. The first weeding was done after 10 days of sowing. At that time, thinning was done for maintenance of a distance of 5-6cm from plant to plant in each row. Second weeding was done after irrigation at 25 days of sowing. Malathion 57EC @ 2ml/L and Rovral-5 WP were sprayed at regular interval to protect crops from aphids and Alternaria blight respectively. Tagging and harvesting of sample plants: At 50% flowering stage, ten plants were selected at random from the 3 middle rows of each plot. The samples were then tagged properly for collecting the data of various morphological characters. Harvesting was done depending upon the maturity of the fruit. Data collection: Data of tagged plant were collected on days required for 50% germination, days required for 50% flowering, pollen fertility (%), plant height at maturity (cm), number of primary branches per plant, number of siliqua per plant, beak length (cm), number of seeds per siliqua, seed yield per per plant (g). For Statistical analysis, the analysis of variance for each character under study was performed by F test. Duncan’s New Multiple Range Test (DMRT) was performed. Genotypic and phenotypic correlation coefficients were estimated using the formula. Path coefficient analysis was done according to the procedure. After calculating the direct and indirect effect of the characters, residual effect (R) was calculated by using the formula.

Source of Information: Int. J. Expt. Agric. 5(2):24-29 (2015)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Seed yield is an end product of interactions of several yield components. Correlation and path coefficients analysis can furnish a basis in determining the relative contribution of each character to seed yield. Hence, from the prevailing probation, it can be concluded that number of seeds/siliqua and number of primary branches/ plant had strong positive association along with high direct effect on seed yield. Therefore, selection of this character would be more effective for yield improvement.

Knowledge Management: Journal