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Regeneration of Plantlets from Grape (vitis Vinifera L.) Through Different Explants

M. M. H. Chouwdhury
Department of Genetic Engineering & Biotechnology, School of Life Sciences, Shahjalal University of Science & Technology, Sylhet-3114, Bangladesh

M. Ashrafuzzaman
Department of Genetic Engineering & Biotechnology, School of Life Sciences, Shahjalal University of Science & Technology, Sylhet-3114, Bangladesh

S. N. Begum
Senior Scientific Officer
Plant Tissue Culture Laboratory, Plant Breeding Division Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh

M. M. Islam
Chief Scientific Officer
Plant Tissue Culture Laboratory, Plant Breeding Division Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh

P. Dhar
Department of Genetic Engineering & Biotechnology, School of Life Sciences, Shahjalal University of Science & Technology, Sylhet-3114, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted at the Plant Tissue Culture Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during the period from March to June 2011 to investigate the effect of different plant growth regulators on growth and plantlet regeneration of grape ‘Zakkao’cultivar by tissue culture technique. Among the different concentration of BAP at 2.5 mg/l supplemented to LS medium was the best response for callus induction and moderate shoot initiation and BAP 2.0 mg/l + 0.5 mg/l NAA for plantlet regeneration, shoot development from shoot tip, node and leaf explants. Maximum shoot height and shoot fresh weight was obtained from the BAP 2.0 mg/l+0.5 mg/l of NAA. For rooting, half strength MS medium with 3.0 mg/l IBA showed best performance in the respect of root length and root fresh weight from node, shoot tip and leaf explants derived callus. An efficient observation found that for in vitro grapevine regeneration, only half strength MS medium without hormonal combination was effective for root and shoot induction from node and shoot tip explants.

Keywords: Growth regulators, BAP, NAA, IBA, LS, MS medium

Location: Plant Tissue Culture Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

Start Date: 00-03-2011

End Date: 00-06-2011

Program Area: Variety and Species

Commodity: Grape

Objectives:

To investigate the effect of different plant growth regulators on growth and plantlet regeneration of grape ‘Zakkao’cultivar by tissue culture technique.

Methodology:

The experiment was conducted at the Plant Tissue Culture Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during the period from March to June 2011. In the present study, node, shoot-tip and leaf segments of a recently introduced grape cultivar named 'Zakkao' were used, the variety 'Zakkao' was collected from Horticulture Development Centre of BADC, Kasimpur. This is an introduced and locally acclimatized variety and now spread all over the country for commercial cultivation. In the present experiment, node, shoot tip and leaf segments of recently introduced grape cultivar named ‘Zakkao’ were used. Before implanting onto culture media these were cut into small pieces for explants. Shoot tip, leaf and nodal segment explants were cultured on ½MS media, LS media and supplemented different concentration and combinations of BAP alone or in combination with NAA or IBA for callus formation, shoot initiation & root regeneration. The first step in the preparation of the medium was the preparation of stock solutions. The various constituents of the medium were prepared into stock solutions for ready use to expedite the preparation of' the medium. Separate stock solutions for macronutrients, micro-nutrients, iron, vitamin, growth regulators etc. were prepared and used. To ensure aseptic condition all instruments, glassware and culture media were sterilized by autoclaving. For the shoot, root and plantlets regeneration the methods are employed that explants prepared by washing and surface sterilization then inoculation, incubation and sub cultured are completed. To investigate the effect of different treatments of the experiment, data were collected on percent of callus induction, Percentage of callus developed Shoots and Percentage of callus developed roots.

Source of Information: Int. J. Sustain. Crop Prod. 7(2):12-18, (2012)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Regeneration ability of grape without hormonal combination was effective for shoot, root induction, proliferation and elongation. Benzylaminopurine (BAP) at 2.5 mg/l in LS medium was effective for callus formation and partially shoots induction but 2.0 mg/l BAP + 0.5 mg/l NAA was most effective for callus formation and shoot regeneration. IBA at 3.0 mg/l in half MS medium was the best for direct callus formation and rooting from explants.

Knowledge Management: Journal