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Performance Study of Different Potato Variety on Meristem Culture

M. H. Khanam
Scientific Officer
A.H.Z. Biotech. Ltd, Padma Residential Area, Vadra, Rajshahi

M. N. A. Chowdhury
Senior Scientific Officer
Spices Research Center, BARI, Shibganj, Bogra, Bangladesh

M. J. Islam
Director
A.H.Z. Biotech. Ltd, Padma Residential Area, Vadra, Rajshahi, Bangladesh

A. C. Das
Assistan professor
Department of Horticulture, Sylhet Agricultural University, Sylhet, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to select the suitable variety through meristem tip culture for potato chips production. Six varieties were used in this study viz. Laddy Rosseta, Astarik, Joity, Kenny Back, Kufri Bahar and Atlanta, at the Biotechnology Laboratory, A.H.Z Biotech Limited, Vadra, Rajshahi, Bangladesh during the period from November 2011 to March 2012. The chemical GA₃ @ 0.25 mg/1 + and NAA @0.02 mg/1 with MS medium were used in this study. It was observed that the variety Joity showed the highest explants survived (54%) and produced shoots and the lowest (39%) was found Atlanta. Days required for shoot appearance was significantly influenced on variety. The minimum (18) days for shoot appearance was found from Joity variety and took maximum days required for the variety of Atlanta (28). In respect of shoot length, node number, shoot diameter and root length always performed better in variety Joity than other variety. It was observed that variety Joity gave the highest fresh weight of shoot (0.75) and root (0.436 g). The highest yield (45 t/ha) and dry matter (28%) were found in Joity. It is concluded that the combination of GA₃ @ 0.25 mg/l + NAA @ 0.02 mg/l performed better for regeneration and multiplication of potato cv. Joity and also yield and dry matter.

Keywords: Potato, Regeneration, Growth regulator, Tip

Location: Biotechnology Laboratory, A.H.Z Biotech Limited, Vadra, Rajshahi, Bangladesh

Start Date: 00-11-2011

End Date: 00-03-2012

Program Area: Postharvest and Agro-processing

Commodity: Potato

Objectives:

To select the best variety through meristem tip culture for potato chips production.

Methodology:

The experiment was conducted to select the suitable variety through meristem tip culture for potato chips production at the Biotechnology Laboratory, A.H.Z Biotech Limited, Vadra, Rajshahi, Bangladesh during the period from November 2011 to March 2012. Six varieties were used in this study viz. Laddy Rosseta, Astarik, Joity, Kenny Back, Kufri Bahar and Atlanta. The experiment was designed in single factor CRD with 5 replications. The chemical GA3 @ 0.25 mg/l + NAA @ 0.02 mg/l with (MS) medium were used in this study. For obtaining meristem tips above mentioned varieties were planted at A.H.Z. Biotech Limited field laboratory at Vadra, Rajshahi, for collecting explants. Shoos tips of different varieties were collected from actively growing twigs and washed under running tap water. Then the shoot tips were disinfected with 0.1% mercuric solution containing approximately 0.02% tween-20 [Polyoxuethelen (20) sorbitan, oleate] for 2 min inside the running laminar air flow cabinet. Treated explants were washed 4-5 times with sterile distilled water to remove the effect of sterilizing agent. Shoot apical meristem consisting of the apical dome with one to two leaf primorida was isolated using sterile hypodermic needle and scalpel under a dissecting microscope . To avoid dehydration, isolated meristems (0.3-0.5 mm) were transferred quickly on the filter paper bridge in test tubes containing sterilized liquid MS medium supplemented with of GA₃ and NAA. After 4-5 weeks, the developed meristems of different varieties were sub-cultured on semi-solid medium with plant growth regulator for next 4-6 weeks for shoot elongation. The data were recorded on explants producing shoots (%), days required to shoot appearance, shoot diameter (mm), root & shoot length (cm), number of node per shoot, fresh weight of shoot and root , maturity days, yield and dry matter. Results were analyzed using MSTAT-C statistical package.

Source of Information: Int. J. Sustain. Crop Prod. 8(1):28-31, (2013)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The variety Joity is the best for regeneration and multiplication of explants through meristem culture (using GA₃ @ 0.25 mg/l + NAA @ 0.02 mg/l with MS medium) and yield with dry matter also. It’s used as a good variety for potato chips production industries.

Knowledge Management: Journal