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Effect of Growth Regulators on Meristem Culture of Potato Cv. Kufri Chips Sona

M. H. A. Khanam
Scientific Officer
A.H.Z. Biotech. Ltd, Padma Residential Area, Vadra, Rajshahi, Bangladesh

M. N. A. Chowdhury
Senior Scientific Officer
Spices Research Center, BARI, Shibganj, Bogra, Bangladesh

M. J. Islam
Director
A.H.Z. Biotech. Ltd, Padma Residential Area, Vadra, Rajshahi, Bangladesh

M. J. Khatun
Ph.D Student
Department of Botany, Rajshahi University, Rajshahi, Bangladesh

M. A. Hussain
Chief Scientific Officer
WRC, BARI, Noshipur, Dinajpur, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted at the Biotechnology Laboratory, A. H. Z. Biotech Limited, Vadra, Rajshahi, Bangladesh during December 2010 to April 2011 to find out a suitable growth regulator combination and concentration of GA₃and NAA with (MS) medium for regeneration of potato cv. Kufri Chips Sona. The treatments were different concentration of GA₃ (0.15, 0.25, 0.35 & 0.45 mg/l) + NAA (0.01, 0.02, 0.03, & 0.04 mg/l). It was found that the highest (53%) explants survived and produced shoots on the combination of GA₃ @ 0.25 mg/l + NAA @ 0.02 mg/l. The highest (8.80 cm) shoots lengths were recorded with combination of GA₃ @ 0.25 mg/l + NAA @ 0.02 mg /l. In respect of node number it was observed that the maximum (8.73) number of node was recorded from GA₃ @ 0.25 mg/l + NAA @ 0.02 mg /l. The highest root length (9.60 cm) was recorded in the treatment of GA₃@ 0.25 mg/l + NAA @ 0.02 mg /l. The explants treated with GA₃ @ 0.25 mg/l + NAA @ 0.02 mg /l gave the highest fresh shoot and root weight (0.738 & 0.350 g, respectively). It is concluded that the combination of GA₃ @ 0.25 + NAA @ 0.02 mg/l performed better for shoot and root regeneration and multiplication of potato cv. Kufri Chips Sona.

Keywords: Potato, Regeneration, Growth regulator (GA3 & NAA), Meristem tip

Location: Biotechnology Laboratory, A. H. Z.Biotech Limited, Vadra, Rajshahi, Bangladesh

Start Date: 00-12-2010

End Date: 00-04-2011

Program Area: Crop-Soil-Water Management

Commodity: Potato

Objectives:

To find out a suitable growth regulator combination and concentration of GA₃ and NAA with (MS) medium for regeneration of potato cv. Kufri Chips Sona.

Methodology:

The experiment was conducted at the Biotechnology Laboratory, A. H. Z Biotech Limited, Vadra, Rajshahi, Bangladesh during the period from December 2010 to April 2011. The treatments were different concentration of GA₃ (0.15, 0.25, 0.35 & 0.45 mg/l) + NAA (0.01, 0.02, 0.03, & 0.04 mg/l). The meristem tips were used in this study. For obtaining meristem tips, potato of Kufri Chips Sona were planted on A. H. Z. Biotech Limited field laboratory at Vadra, Rajshahi, for collecting explants. Shoot tips were collected from actively growing twigs and washed under running tap water. Then the shoot tips were disinfected with 0.1% mercuric chloride solution containing approximately 0.02% tween-20 [polyoxuethelen (20) sorbitan, oleate] for 2 minute inside the running laminar air flow cabinet. Treated explants were washed 4-5 times with sterile distilled water to remove the effect of sterilizing agent. Shoot apical meristem consisting of the apical dome with one to two leaf primorida was isolated using sterile hypodermic needle and scalpel under a dissecting microscope. To avoid dehydration, isolated meristems (0.3-0.5 mm) were transferred quickly on the filter paper bridge in test tubes containing sterilized liquid MS medium supplemented with different concentrations of GA₃ and NAA . After 4-5 weeks, the developed meristems were subcultured on semi-solid medium with different levels of plant growth regulator for next 4-6 weeks for shoot elongation. The recorded data were on explants producing shoots (%), shoot length (cm), number of node per shoot, root length (cm), fresh weight of root (g) and shoot (g). The experiment was designed in single factor CRD with 5 replications. Results were analyzed using MSTAT-C statistical package.

Source of Information: Int. J. Sustain. Crop Prod. 8(1):22-24, (2013)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the above study it is occluded that the combination of GA₃ + NAA (@ 0.25 & 0.02 mg/l respectively is the best for shoot and root regeneration and multiplication of potato cv. Kufri Chips Sona in comparison to the others combination of GA₃ + NAA with MS medium.

Knowledge Management: Journal