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Effect of Inoculation Methods of Rhizobium on Yield Attributes, Yield and Protein Content in Seed of Pea

R. Ahmed
Scientific Officer
Soil & Water Management Section, Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI) Joydebpur, Gazipur-1701

A. R. M. Solaiman
Professor
Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706

N. K. Halder
Senior Scientific Officer
Soil & Water Management Section, HRC, BARI Joydebpur, Gazipur-1701

M. A. Siddiky
Scientific Officer
Soil & Water Management Section, HRC, BARI Joydebpur, Gazipur-1701

M. S. Islam
IPM Field organizer
IPM Project, BARC, Bangladesh

Abstract/ Executive Summary:

A field experiment was conducted at the experimental farm of the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during November, 2003 to March, 2004 to evaluate different methods of Rhizobium inoculation on yield attributes, pod and seed yields and protein content in seed of pea (Pisum sativum) cv. IPSA Motorshuti-3. Seed or soil or both seed and soil were inoculated either with Rhizobium strain BARI RPs-2001 or BARI RPs-2002. The performances of Rhizobium strains BARI RPs-2001 and BARI RPs-2002 used as seed or soil inoculation were superior to uninoculated control in all the parameters of the crop. Among the treatments, seed and soil inoculation with Rhizobium strain BARI RPs-2002 performed best in recording number of pods/plant, number of seeds/pod, 1000- seed weight, pod and seed yields and protein content in seed of pea plants. The highest green pod yield (3.29 t/ha), green seed
yield (1.90 t/ha), mature pod yield (1.03 t/ha) and mature seed yield (0.76 t/ha) were obtained by coinoculation of seed and soil with Rhizobium strain BARI RPs-2002 showing 53, 58, 91 and 100% increase in yield over uninoculated control, respectively. Coinoculation of both seed and soil with Rhizobium strain BARI RPs-2001 was similar to the effect of the treatment receiving seed and soil inoculation with Rhizobium strain BARI RPs-2002. There was a positive correlation among yield attributes and pod and seed yields and protein content in seed of pea. From the viewpoint of yield attributes and yield of pea, coinoculation of both seed and soil with Rhizobium strain BARI RPs-2002 was considered to be an effective treatment for achieving the maximum output through cultivation of pea in Shallow-Red Brown Terrace soil.

Keywords: Rhizobium, Yield attribute, Protein content

Location: Experimental farm of the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur

Start Date: 00-11-2003

End Date: 00-03-2004

Program Area: Postharvest and Agro-processing

Commodity: Pea

Objectives:

To find out the effect of inoculation methods of Rhizobium on yield attributes, yield and protein content in seed of pea

Methodology:

A field experiment was conducted during the Rabi season covering the period of November, 2003 to March, 2004 at the experimental farm of the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur with a view to find out the effect of inoculation methods of Rhizobium on yield attributes, yield and protein content in seed of pea. The soil of the experimental farm was Shallow-Red Brown Terrace soil under Madhupur Tract (AEZ No. 28). It was of clay loam texture and contained 0.73% organic carbon, 12.05 (meq/100g dry soil) CEC, 0.074% total N, 12.05 ppm available phosphorus and had a pH 6.4. The number of Rhizobium per g of soil was 5.9 × 10⁴. The experiment was laid out in a Randomized Complete Block Design (RCBD) with four replications. The unit plot size was 2.4 m×2.4 m. There were eight treatment combinations in the experiment which were uninoculated control (T₁), seed inoculation with Rhizobium strain BARI RPs-2001 (T₂), soil inoculation with Rhizobium strain BARI RPs-2001 (T₃), seed + soil inoculation with Rhizobium strain BARI RPs-2001 (T₄), seed inoculation with Rhizobium strain BARI RPs-2002 (T₅), soil inoculation with Rhizobium strain BARI RPs-2002 (T₆), seed + soil inoculation with Rhizobium strain BARI RPs-2002 (T₇) and 50 kg N/ha (T₈ ). At the time of final land preparation a basal dose of 60 kg P₂O₅, 50 kg K₂O and 20 kg S/ha was applied to each plot. Nitrogen at the rate of 50 kg /ha was used in the form of urea as per treatment. Pea (var. IPSA motorshuti-3) was used as the test crop for the experiment. The study was carried out with the Rhizobium inoculant containing strains viz. BARI RPs-2001 and BARI RPs-2002. The inoculants were collected from the Soil Science Division of the Bangladesh Agricultural Research Institute (BARI). Rhizobial population of the inoculants containing BARI RPs-2001 and BARI RPs-2002 were 1.85 x 108cell/g and 4.75 x 108cell/g, respectively as estimated following the method of Miles and Misra. Seeds were taken in vinyl bags and then gum arabic solution was added to the seeds to make them sticky. Inoculants were used for inoculating seeds at the rate of 20g/kg of seed. The seeds were mixed with the inoculants thoroughly. After inoculation, the seeds were air-dried by spreading them on a polyethylene paper placed on the table. Liquid inoculant was prepared by making a suspension of peat based inoculant in distilled water and then 210g inoculant was suspended in 600 L water. Soil was inoculated with liquid inoculant at the rate of 3000 L/ha where necessary. Seeds were sown in each plot at the rate of 35kg/ha. Pods were harvested at two different stages of maturity. During harvesting stage the number of pods/plant and number of seeds/pod were recorded from five randomly selected plants from each plot. Green pod was harvested and estimated from the yield of plants grown in half portion of each plot. The rest half of the crop of each plot was kept to allow the pods to get maturity. Then the matured pods were harvested and estimated from the yield of plants grown in the remaining half portion of each plot. Thousand seed weight was estimated from both green and mature seeds. The seeds were first air-dried and then oven-dried at 65⁰C for 72 hours. Then seeds were powdered by a grinding machine for nitrogen determination. Total nitrogen content in seed was determined by Micro-Kjeldahl method following Salicylic-H₂SO₄digestion through colorimetric method. N was measured using double beam spectrophotometer (Model No. 200-20, Hitachi, Japan) at 625 nm wave length. The crude protein content was estimated by multiplying the N% determined with a factor of 6.25. The recorded data on various characters of the crop were statistically analyzed to find out the significance of variation resulting from the experimental treatments. For this purpose, analysis of variance was worked out for each character of the crop. The differences between treatment means were compared by Duncan’s Multiple Range test (DMRT).

Source of Information: J.Soil.Nature. 1 (3):30-35, (2007)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

There was a positive correlation among yield attributes and pod and seed yields and protein content in seed of pea. From the viewpoint of yield attributes and yield of pea, coinoculation of both seed and soil with Rhizobium strain BARI RPs-2002 was considered to be an effective treatment for achieving the maximum output through cultivation of pea in Shallow-Red Brown Terrace soil.

Knowledge Management: Journal