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Mass Production of Ompok Pabda Seed by Dose Optimization of Ovaprim and Current Status of Ompok Pabda Farming in Jamalpur Sadar

Antara Ghosh Chaity
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

An experiment was conducted for mass production of Ompok pabda seed through induced breeding using S-GnRH/Ovaprim and data was collected to know the current status of O. pabda culture in Jamalpur Sadar Upazilla. Three treatments were designed with various doses of hormone to find out the most effective one. The treatment were designated as T1 (18 ml/kg body weight), T2 (20 ml/kg body weight) and T3 (25 ml/kg body weight) for female. In case of males, for all treatment hormone dose of 12.5 ml/kg body weight of fish was used. After ending the trial period, among three doses T3 showed the best results in terms of fertilization rate (88±4.0%), hatching rate (69±3.61%), and survival rate (64.67±2.52%). While, lowest fertilization rate (77±7.0%), hatching rate (38±7.0%), and survival rate (23±2.0%) was found in T1. In order to understand the present status, of culture practice of O. pabda questionnaires interview schedule were used. Among the 22 hatcheries in Jamalpur district 8 hatcheries situated in Jamalpur Sadar, only one hatchery (Johurul Matshya Khamar) practiced production of O. pabda seed through induced breeding. About 18 farmers were practicing only O. pabda culture in this Sadar Upazilla. Data was collected from 8 hatchery owners and from 18 fish farmers then analyzed. The annual income of O. pabda fish farmers ranged from TK.120000-250000. Therefore, production of Ompok pabda seed and their culture practice were beneficial both for the hatchery owners and the fish farmers.

Keywords: Production ,Ompok pabda, Ovaprim, Jamalpur sadar

Location: Jamalpur Sadar Upazilla

Start Date: 00-05-2013

End Date: 00-07-2013

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

1. To know the seed production technique of O. pabda in a private hatchery.

2. To optimize the doses of Ovaprim for induced breeding.

3. To know the present status of O. pabda culture in Jamalpur Sadar.

Methodology:

Study area: The whole research experiment was conducted in Johurul Matshya Khamar. The hatchery is situated in west Fulbaria road (P.T.I road) Jamalpur, a successful private hatchery in this region having a national Award in Agriculture. Total area of hatchery about 13 acres. Brood stock collection and rearing technique: Proper management of brood fish is important task for getting desired seed production. Only healthy brood fish can give healthy fry. Ripe broods will be available during the whole breeding season when brood stock maintained properly. Brood of O. pabda reared in brood pond having 20 decimal areas with an average depth of 3.5 feet. A total of about 500 O. pabda both male and female were collected from Bangladesh Fisheries Research Institute (Mymensingh) and stocked in previously prepared pond during May 2013. Brood stock management was initiated in early June and continued up to the completion of breeding activity up to July. Formulated feed are given during early January for proper gonadal development of brood fish. Predators and unwanted fishes are controlled manually by using net. Control of aquatic vegetation also practiced properly in brood fish pond. Feed were applied at a rate of 4% body weight twice daily once in morning and then in evening. Cowdung was used for manuring at a rate of 5 kg/decimal. Fertilization was done with 200 mg/decimal urea and 100 mg/decimal TSP. Sampling procedure: Sampling was done to know the health condition of the fishes as well as to determine the amount of daily ration. Sampling was performed at monthly interval from May to June 2013. The Weight (g) was recorded by random sampling of 20 fishes from pond by using a seine net. Experimental design: Three breeding trials were performed for dose optimization with ovaprim hormone. Three hapas made of glass nylon each having a size of 2.1×1.5×1 m3 were used and marked as T1, T2 and T3 hapa and 40 brood fish (20 male+ 20 female) were kept in each hapa after hormone treatment. Hormone preparation: To prepare Ovaprim hormone solution subsequent amount of distilled water was added and then taken in 1 ml hypodermic syringe. Injecting broods: Broods were collected from hapa just prior to injection. A single injection was given to both male and female from three doses of Ovaprim hormone. The broods were treated with inducing agent at evening so that fish ovulate in morning. During administration of injection fish were wrapped by a soft and wet cloth and kept lying on soaked foam. The Ovaprim solutions were injected intramuscularly at dorsal side behind pectoral fin. The needle pushed about 45ºangle to the body surface during the time of injection. Fertilized egg collection: Hormone treated brood fish released egg and milts within 9 to 10h and 12h require for fertilization then fish were removed from hapa. Fertilized eggs were transferred into the new hapa maintained with continuous water flow from porous pipe for aeration. Hapa was filled with filtered pond water to minimize the temperature difference and environmental shock. Fertilization rate (%): Fertilization rate can be counted by direct counting method. For this purpose bowl of 2 liter capacity were used. Three replications were used for each sampling in a day. The average values of fertilized eggs of three bowls were determined by using following formula. For determination of fertilization and hatching rate approximately 100 eggs were placed in a bowl. After fertilization transparent eggs were considered as fertilized egg and opaque eggs considered as dead eggs. Hatching rate (%): Hatching rate were counted by direct counting method. Hatchling were counted by taken in white enamel bowl which was capacity of 2 liter then water containing hatchling were shifted to another bowl by siphoning method. The average value of three bowls was calculated. Survival rate (%): Three days after fertilization hatchlings were transferred to hatchling tray and after five days first feed were given to hatchling twice daily with boiled chicken egg. One egg was given to per kg of fry at 6 h interval. The larvae were always fed to satiation level. They were considered satiated when they stopped searching feed and assembled under the shelter or in the side of hapa. Two third of water was exchanged as well as the hapa were cleaned and the dirt of the bottom were removed once daily in morning. Same water level was maintained in all three hapa. During the whole experimental period, the dead larvae were removed and the numbers of dead larvae were recorded. Sampling was done at every 7 days interval and 10 larvae were randomly collected from each hapa and weighed in a petridish which was set on a 1 mm graph paper and readings were obtained in millimeters. Samplings were usually done before application of feed to avoid the biasness in weight due to presence of excessive feed. After recording the length and weight of the larvae they were released in the respective hapa. After 7 days the total number of live larvae in each hapa was counted separately by taken in 2 liter bowl for calculation of survival rate. The present status of Ompok pabda culture in Jamalpur Sadar Upazilla: Selection of area for data collection The study was conducted in Jamalpur sadar. Jamalpur Sadar Upzilla with its 15 union namely Kendua, Sharifpur, Lakshmir char, Tulsir char, Ranagachha, Narundi, Ghoradhap, Titpalla, Sahabajpur, Meshta, Digpaith, Banshchara, Sreepur, Rashidpur were selected as the specific study locations based on the availability of number of farmer involved in pabda farming. Identify target group: The hatchery owners and pabda farmers in Jamalpur Sadar were the target population of the study. Instrument for data collection: A structured interview schedule was used as the data gathering instrument. The schedule was prepared in Bangla for clear understanding of the respondents. It may be recalled that the draft interview schedule was pretested among 15 respondents of the study area before using the same for final data collection. Necessary correction, modification and additions were made in the interview schedule on the basis of results of pre-test. The interview schedule was than printed in its final from. Collection of data: The researcher herself collected data from the sampled farmers by using the personal interview schedule during the month of June, 2013. Before starting collection of data, the researcher meets the respective District Fisheries Officer, Upazilla Fisheries Officer (UFO), and Fisheries Extension Officer (FEO) and Farmers of the Jamalpur Sadar Upazilla. Data processing and analysis: The fertilization (%), Hatching rate (%) and Survival rate (%) were tested using one way analysis of variance (ANOVA) and collected questionnaire interview data were coded, compiled, categorized, tabulated and analyzed. Qualitative data were converted to numerical values by the means of suitable scoring techniques. The SPSS (Statistical Package for Social Sciences) computer package was used to perform data analysis. Descriptive statistics such as mean, range, number and percentage, standard deviation and rank order were used whenever necessary mentioned before and were transferred to a master sheet to facilitate tabulation. Local units were also converted into standard units.

Source of Information: MS Thesis, Roll No. 13 Fish Aqua JJ-11 M Registration No. 35773, Session 2008-09, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The fish responded to hatch by a single Ovaprim dose 12.5 ml/kg for male and for female ranges from 18-25 ml/kg body weight of fish among which 25 ml/kg gave the best result in consideration of fertilization, hatching and survival rate. The highest average fertilization rate 88±4.0% was recorded in T3 (25 ml/kg/bw) dose of Ovaprim whereas lowest average fertilization rate 77±7.0% were recorded in T1 (18ml/kg/bw). The highest hatching rate was recorded as (69±3.6%) in T3 and lowest hatchling rate was recorded a 38±7.0% in T1. The highest survival rate of O. pabda was 64.67±2.5% in T3 whereas lowest survival rate (23±2.0%) was found in T1. The overall hatching rates were not very high which may be considered as an impediment towards producing large quantity of seeds. Successful stripping at an appropriate post-ovulation period could minimize the problem to some extent. Further experimentation is required to pin-point the stripping time after ovulation. Another study based on questionnaire interview on Pabda aquaculture in Jamalpur sadar. Collection of data was conducted from June to April. There were around 8 hatcheries at Jamalpur Sadar Upazilla, maximum of which were involved in carp seed production. In Johurul Matshya Khamar O. pabda seed were produced. There were about 18 farmers involved in Pabda aquaculture practice having average pond size of 0.16 ha, the highest annual income of the farmers about Tk.250000 whereas lowest income was about 28000. Based on the market demand culture practice of this species had been flourished. But, some problems were evident in the present study among them the most important was lack of seed supply and another was lack of technical knowledge. To increase the production of O. pabda, it is important to increase the production of seed and necessary information should be provided to the farmer for making them interested in this aquaculture practice.

Knowledge Management: Thesis