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Antioxidant Potential and Nutrient Content in Selected Fish Species of Different Feeding Habits in Bangladesh

M. Golam Sarower
Fisheries& Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh

Sunuram Ray
Centre for Integrated Studies on the Sundarbans, Khulna University, Khulna -9208, Bangladesh

Md. Abir Hasan
Fisheries& Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh

Subarna Ferdous
Fisheries& Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh

M. Iqbal Ahmed
Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh

M. Muslima Khatun
Fisheries& Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh

Abstract/ Executive Summary:

This study reports the antioxidant potential and chemical composition of muscle from 30 fish species of different feeding habits namely carnivore, herbivore and omnivore.Different in vitro assays used for determining antioxidant potential of extracts of fish species were: thin layer chromatography (TLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. No significant difference of antioxidant activity or nutrient content was observed among carnivorous, herbivorous and omnivorous fishes. The IC₅₀ measured by DPPH method varied from 154 to 2139 μg/ml of wet weight. The lowest IC₅₀ value was observed in C. idella followed by P.ticto, C. striatus, C. punctatus, L. rohita, A. testudineus, M. cordyla, O. mossambicus and G. giuris. The moisture content of samples ranged from 62 - 85%. The protein content was high in C. idella (24.3%) and low in L. calbasu (10.57 %). The lipid and ash content analyzed in the selected fish species ranged from 1.17-7.94% and 1.31-4.80%, respectively. Overall, the results suggest that fish of different feeding habit can be exploited for their antioxidant and nutrients components and used for consumption as well as value addition in food formulations.

Keywords: Antioxidant activity, DPPH free radical scavenging, Proximate composition, Herbivore, Carnivore,

Location: Khulna University

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Fish

Objectives:

To report the antioxidant potential and chemical composition of muscle from 30 fish species of different feeding habits namely carnivore, herbivore and omnivore.

Methodology:

Sample collection and preparation: On the basis of different habits, 30 fishes classified into three groups as herbivore, carnivore and omnivore were purchased from different fish markets in Khulna, Bangladesh. After washing with distilled water, the muscle from collected fishes was separated from fish body by a sharp blade except A. mola. Because of smaller size whole A. mola was used in sample preparation. Then 100 g fish sample was homogenized in ethanol and filtered it. The filtrate was kept in the shaking water bath at 40 ºc for drying. The fish extract and ascorbic were taken in a small vial and serial dilutions (0, 1, 50, 100, 200, 300, 400 and 500 μg/ml)were prepared in ethanol and 0.004% DPPH were added to measure free radical scavenging activity. In this study, ascorbic acid was used as a reference standard antioxidant. The samples were analyzed for antioxidants, moisture, protein, lipid and ash in triplicate. Estimation of antioxidant activity: Initially antioxidant activity was determined by TLC method. After applying DPPH on the TLC plates, yellow or whitish color on purple background was observed in the ethanol extracts of fishes. Yellow or pale yellow color indicated the presence of antioxidant components in the sample. Then, radical scavenging activity of fish extracts against stable DPPH was determined by the slightly modified method. Freshly prepared DPPH solution (0.004% w/v) was taken in the test tubes, then extracts (stock solution) were added to the tubes and shaken vigorously so that the final volume would be 3 ml. In the dark condition the tubes were allowed to stand for 30 min for the reaction to occur. The absorbance was determined at 517 nm using a spectrophotometer (HITACHI U-2910). First, the % inhibitions of DPPH free radical was measured, then % inhibitions were plotted against concentration and the inhibitory conc. 50% (IC₅₀)was measured. Analysis of proximate composition: Moisture:Moisture was determined by complete drying of the sample at 105⁰ C in an oven. The percentage of the moisture content was calculated. Statistical methods: The results were expressed as mean±SD. T-test was used to examine the difference between antioxidant levels and proximate composition of fish samples.

Source of Information: Am. J. PharmTech Res. 2014; 4(4) pp. 387-397. ISSN: 2249-3387

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The lowest IC₅₀ value was observed in C. idella followed by P.ticto, C. striatus, C. punctatus, L. rohita, A. testudineus, M. cordyla, O. mossambicus and G. giuris. The moisture content of samples ranged from 62 - 85%. The protein content was high in C. idella (24.3%) and low in L. calbasu (10.57 %). The lipid and ash content analyzed in the selected fish species ranged from 1.17-7.94% and 1.31-4.80%, respectively. Overall, the results suggest that fish of different feeding habit can be exploited for their antioxidant and nutrients components and used for consumption as well as value addition in food formulations.

Knowledge Management: Journal