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In Vitro Rapid Regeneration from Cotyledon Explant of Native-olive (elaeocarpus Robustus Roxb.)

M.M. Rahman
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

M.N. Amin
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

R. Ahmed
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Abstract/ Executive Summary:

In the present study an efficient tissue culture method for high rate of shoot regeneration was developed for Elaeocarpus robustus. Cotyledonary explants with and without petiolar end were cultured in _½MS medium supplemented with auxin and cytokinin. Explant with the petiolar/proximal end showed a better response than those without it in respect of shoot initiation. Half strength of MS medium supplemented with 1.0 mg l^-1 BA+0.5 mg l^-1 2,4-D was found to be best for producing organogenic callus. The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l^-1 BA+0.5 mg l^-1 Kn+0.1 mg l^-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them _½in MS medium supplemented with 0.2 mg l^-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.

Keywords: In vitro, Cotyledon explant, Regeneration, Olive, Tissue culture

Location: Department of Botany, University of Rajshahi, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Indian olive

Objectives:

To describes organogenesis and plant regeneration from cotyledon-derived callus cultures of native-olive.

Methodology:

Seeds of Elaeocarpus robustus were collected from mature fruits and cotyledons were excised from these seeds. Excised cotyledons were washed in distilled water and then surface sterilized with 0.1% HgCl₂ solution for 5 min. followed by thorough washing with sterilized distilled water. Two types of explants namely, cotyledon with petiolar / proximal end and without petiolar end / with distal end were cultured in _½MS medium supplemented with different combinations of BA, Kn and 2,4-D for callus induction. After six weeks, calli were subcultured in conical flasks containing different combinations of cytokinin and auxin supplemented media for shoot induction. Multiple shoots developed in the medium containing 1.0 mg l^-1 BA+0.5 mg l^-1 Kn+0.1 mg l^-1 NAA. Elongated shoots were excised, made into microcuttings (2-3 cm) and transferred to _½MS medium with 0.1-1.0 mgl^-1 of either IBA, NAA or IAA for rooting. All media were gelled with 0.7% agar (BDH) and supplemented with 30 mg l^-1 sucrose. The pH of the medium was adjusted to 5.7±0.1 prior to autoclaving at 121°C under 1.1 kg cm^-2 pressure for 20 min. The cultures were maintained in a growth room under 16 h photoperiod at 26±1°C.

Source of Information: Asian Journal of Plant Sciences Volume 3, Number 1, 31-35, 2004, ISSN 1682-3974

Article Link (Online Journal): DOI: 10.3923/ajps.2004.31.35, URL: http://scialert.net/abstract/?doi=ajps.2004.31.35

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l^-1 BA+0.5 mg l^-1 Kn+0.1 mg l^-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them _½in MS medium supplemented with 0.2 mg l^-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.

Knowledge Management: Journal