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In Vitro Rooting and Ex Vitro Plantlet Establishment of BARI Banana 1 (musa Sp.) as Influenced by Different Concentration of Iba (indole 3-butyric Acid)

M.M.H. Molla
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

M. Dilafroza Khanam
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

M.M. Khatun
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

M. Al-Amin
Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

M.A. Malek
Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

Abstract/ Executive Summary:

Shoot tip culture of BARI banana 1 (Musa sp.) were cultured on MS medium supplemented with 5.0 mg L^-1 BAP for shoot proliferation. Well-developed shoots were used for rooting. Among the six different concentrations of IBA (0.1, 0.2, 0.3, 0.4, 0.5, 0.6 mg L^-1) with half strength MS medium, a good number of healthy roots were produced on half MS+0.5 mg L^-1 IBA (7.86) followed by half MS+0.6 mg L^-1 IBA (6.89) and half MS+0.4 mg L^-1 IBA (6.31) with the weight of 0.85, 0.83 and 0.77 g plantlet^-1, respectively. However, 95-100% plantlets were survived when they were transferred to small plastic pots after 15-20 days in vitro culture on half MS medium supplemented with 0.4–0.6 mg L^-1 IBA and 7 days hardening at room temperature.

Keywords: Banana, In vitro rooting, Hardening, IBA concentration, Biotechnology

Location: Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Banana

Objectives:

To standardize IBA concentration and days for in vitro rooting with 7 days hardening at room temperature for better plant survival of BARI banana-1 in ex vitro condition.

Methodology:

BARI Banana 1 (Musa sp.) is a high yielding and newly released banana variety in Bangladesh. Shoot tips were collected from the virus free sword sucker of the variety. Roots and outer layer of tissues of the suckers were removed and the remaining portions were washed with tap water using detergent. The explant then surface sterilized in 0.1% mercuric chloride (HgCl₂) with a few drops of Tween 20 for 30 min. After washing 4 times with sterilized distilled water, the shoot tips were further cut to a size of approximately 5x8 mm portion containing an intact apex under clean bench. The explants were placed on MS solid medium with 5.0 mg L^-1 BAP. All cultures were incubated at 25±1°C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes. The pH of the medium was adjusted to 5.8 prior to autoclaving. The materials were sub-cultured at 30 days interval in the same medium to produce multiple shoots. Well-developed shoots were transferred to rooting medium containing half MS medium supplemented with different concentrations of IBA (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 mg L^-1). Each treatment contained ten replicate with six shoots/replicate. Every five days interval plantlets from different concentrations, transferred at room temperature for seven days hardening. After that, the plantlets were washed in water carefully to remove the media and transferred to small plastic pots containing a mixture of sterilized soil and sand for five days where high humidity was maintained by covering the plantlets with polyethylene sheet. The plantlets were then planted in poly bags (12.5 x 17.5 cm) containing soil, sand and cowdung (1:1:1) and were kept in nethouse. After incubation in rooting medium every 5 days interval, number of roots were recorded. After 15 days in vitro culture with 7 days hardening at room temperature average number of roots, length of roots, fresh weight of roots, number of leaves and length of shoots were recorded.

Source of Information: Asian Journal of Plant Sciences Volume 3, Number 2, 196-199, 2004, ISSN 1682-3974

Article Link (Online Journal): DOI: 10.3923/ajps.2004.196.199, URL: http://scialert.net/abstract/?doi=ajps.2004.196.199

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results indicated that 95-100% plantlets survived when they were transferred after 15 -20 days in vitro culture on 0.4-0.6 mg L^-1 IBA concentration with 7 days hardening at room temperature.

Knowledge Management: Journal