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In Vitro Micropropagation of Some Important Sugarcane Varieties of Bangladesh

M.A. Mamun
Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208, Bangladesh.

M.B.H. Sikdar
Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208, Bangladesh.

Dipak Kumar Paul
Department of Applied Nutrition and Food Science, Islamic University, Kushtia-7003, Bangladesh.

M. Mizanur Rahman
Department of Biotechnology, Islamic University, Kushtia-7003, Bangladesh.

Md. Rezuanul Islam
Department of Biotechnology, Islamic University, Kushtia-7003, Bangladesh.

Abstract/ Executive Summary:

In vitro micropropagation of sugarcane variety viz., Isd-28 and Isd-29 were studied. Nutrient media containing growth regulators, enhanced callus induction, shoot differentiation and root formation in vitro were the elements investigated. For the experiment, surface sterilization of the explants from the field grown plants was essential and sterilization with 0.1% HgCl₂ for 8 min was satisfactory. In this treatment, 85-90% of the leaf sheath explants was contamination free. Among the explants cultured for proliferation fortified with 3.0 mg L^-1 2, 4-D explants of all the 2 varieties of sugarcane showed the best performance for the callus induction after 16-20 days of culture. The concentration of BA tested 1.5 mg L^-1 of the cytokinin produced comparatively higher percentage of shoot proliferation. Different concentration of BA mg L^-1 in combination with different auxin viz., IAA, IBA and NAA were tested for shoot proliferation. Isd-28 and Isd-29 showed best shooting when media were supplemented with BA 1.5 mg L^-1+0.5 mg L^-1 NAA. In vitro proliferated shoots were rooted on MS and modified MS media. Best results of rooting ware observed on modified MS medium supplemented with auxins (NAA+IBA) 0.5 mg L^-1 and 0.5 mg L^-1 showed fair results. Eighty to ninety percent regenerated plantlets were viable at normal temperature with 85% humidity while transferred sterilized soil.

Keywords: Auxins, Cytokinin, Callus induction, Surface sterilization, Sugarcane varieties

Location: Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

1. To determination of the most suitable medium compositions for rapid response on callus induction,

2. To find out appropriate concentration and combination for inducing roots in vitro, and establishment of plantlets.

3. To observe the variation of in vitro grown sugarcane plant for breeding program.

Methodology:

The young leaf-sheath of two sugarcane varieties viz., Isd-28 and Isd-29 were used as an experimental material. The explants were collected from 2 months old field grown plants, raised from the stem cutting. These explants were surface sterilized with various percentages of mercuric chloride (HgCl₂) and inoculated aseptically for in vitro regeneration on MS or modified MS medium with different concentrations and combinations of growth regulators. Media for callus induction and there multiplication, different strengths of MS media supplemented with mesoinositol as organic compound as well as different concentration of auxin (2,4-D) and coconut milk were used, shoot induction media/Media for shoot differentiation from callus tissue, MS medium supplemented with auxins (NAA, IBA), cytokinins (BA) and coconut water were used for the purpose, media for root formation in vitro growing shoot, modified medium supplemented with different concentrations and combinations of auxins (NAA, IBA) and Activated Charcoal (AC) were used for the rooting of the shoots.

Source of Information: Asian Journal of Plant Sciences Volume 3, Number 6, 666-669, 2004

Article Link (Online Journal): DOI: 10.3923/ajps.2004.666.669 URL: http://scialert.net/abstract/?doi=ajps.2004.666.669

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Best results of rooting were observed on modified MS medium supplemented with auxins (NAA+IBA) 0.5 mg L^-1 and 0.5 mg L^-1 showed fair results. Eighty to ninety percent regenerated plantlets were viable at normal temperature with 85% humidity while transferred sterilized soil.

Knowledge Management: Journal