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Evaluation of Thrombolytic and Membrane Stabilizing Activities of Four Medicinal Plants of Bangladesh

S.R. Chowdhury
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

T. Sharmin
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

M. Hoque
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

Md. Sumsujjaman
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

M. Das
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

F. Nahar
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

Abstract/ Executive Summary:

An experiments were conducted in Phytochemical Research Laboratory, State University of Bangladesh in 2012.The crude methanol extracts of aerial parts of Abrus precatorius L., leaf of Magnolia pterocarpa Roxb. and Dracaena spicata Roxb. and leaf and bark of Ravenala madagascariensis Sonn. as well as their hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screenings for thrombolytic and membrane stabilizing activities. Among the extractives of A. precatorius, the crude methanol extract exhibited the highest thrombolytic activity (34.92±0.54 %) while the carbon tetrachloride soluble fraction of M. pterocarpa exhibited 22.59±0.88 % clot lysis. D. spicata extractives showed mild thrombolytic activity. The methanolic crude extract of R. madagascariensis leaf and the aqueous soluble fraction of R. madagascariensis bark extract showed 45.32±0.82 % and 32.67±0.74% clotlysis, respectively. In hypotonic solution and heat induced conditions, the crude methanol extract of A. precatorius and the hexane soluble fraction of crude methanol extract of M. pterocarpa inhibited 63.46±0.84 % & 36.54±0.21 % and 66.12±0.66 % & 40.54±0.02 % haemolysis of RBCs, respectively as compared to 71.90 % and 42.12 % inhibition by acetyl salicylic acid (0.10 mg/ml), respectively. The crude methanol extract of D. spicata demonstrated 64.44±0.68 % and 36.52±0.19 % inhibition of hypotonic solution and heat induced hemolysis, respectively. The chloroform soluble fraction of R. madagascariensis leaf extract demonstrated 28.72±0.61 % & 39.97±0.39 % and the hexane soluble fraction of R. madagascariensis bark extract revealed 53.78±0.17 % & 41.83±0.61 % inhibition of hypotonic solution and heat induced hemolysis of RBCs, respectively.

Keywords: Abrus precatorius, Magnolia pterocarpa Roxb., Dracaena spicata Roxb., Ravenala madagascariensis Sonn., Thrombolysis, Membrane stabilization

Location: Phytochemical Research Laboratory, State University of Bangladesh

Start Date: 00-00-2012

End Date: 00-00-2012

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To evaluate the thrombolytic and membrane stabilizing potentials of crude methanol extracts of A. precatorius, leaf of M. pterocarpa and D. spicata and leaf and bark of R. madagascariensis as well as their hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates.

Methodology:

The experiments were conducted in Phytochemical Research Laboratory, State University of Bangladesh in 2012. The aerial parts of A. precatorius, leaf of M. pterocarpa and D. spicata and leaf and bark of R. madagascariensis were collected in March 2012 from Dhaka. Voucher specimens DUSH-10775, DUSH-10774 and DUSH-10777 for collection of A. precatorius, M. pterocarpa and D. spicata have been deposited in Salar Khan Herbarium, Department of Botany, University of Dhaka, respectively. In Bangladesh National Herbarium, voucher specimen DACB 38302 has been deposited for the collection of leaf and bark of R. madagascariensis. The collected plant materials were cleaned, sun dried and pulverized. The powdered materials (500 g each) of the collected plants were separately soaked in 2.0 liters of methanol at room temperature for seven days. The extracts were then filtered through fresh cotton bed and finally with Whatman filter paper number 1 and concentrated with a rotary evaporator at reduced temperature and pressure. An aliquot (5 g) of each of the concentrated methanol extract was fractionated by the modified Kupchan partition protocol and the resultant partitionates were evaporated to dryness with rotary evaporator to yield hexane (HXSF), carbon tetrachloride (CTCSF), chloroform (CSF) and aqueous (AQSF) soluble materials. The thrombolytic activity was evaluated by the method developed by Prasad et al (2006) by using streptokinase as positive control.The membrane stabilizing activity of the extractives was assessed by evaluating their ability to inhibit hypotonic solution and heat induced haemolysis of human erythrocytes following the method developed by Omale et al (2008). For all bioassays, three replicates of each sample were used for statistical analysis and the values were reported as mean ± SD.

Source of Information: International Journal of Pharmaceutical Sciences and Research, 2013; Vol. 4(11): 4223-4227.

Article Link (Online Journal): http://dx.doi.org/10.13040/IJPSR.0975-8232.4(11).4223-27

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The extractives of A. precatorius, M. pterocarpa, D. spicata and R. madagascariensis bark exhibited mild to moderate thrombolytic activity but the R. madagascariensis leaf extractives demonstratd significant thrombolytic activity. On the other hand, the extractives of A. precatorius, M. pterocarpa, D. spicata and R. madagascariensis bark exhibited significant membrane stabilizing activity but the R. madagascariensis leaf extractives demonstrated mild to moderate membrane stabilizing activity. Therefore, these plants are good candidates for further systematic, chemical and biological studies to isolate the active principles.

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