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Comperative Efficacy of Three Indigenous Medicinal Plants (turmeric, Garlic, and Aloe) with Locally Available Patent Drug Nebanol® Against Artificially Induced Wound in Guinea Pigs

Mohammad Abu Taher
Lecturer
Faculty of Unani and Ayurvedic Medicine, Hamdard University Bangladesh (HUB), New town Sonargaon, Narayanganj. Bangladesh.

Hasib Sheikh
Faculty of Unani and Ayurvedic Medicine, Hamdard University Bangladesh (HUB), New town Sonargaon, Narayanganj. Bangladesh.

Md Shahinoor Rahman Dulal
Routi Herbal Limited and Routi Pharmaceuticals (Unani) Limited. Dhaka, Bangladesh

Md. Khairul Alam
Faculty of Unani and Ayurvedic Medicine, Hamdard University Bangladesh (HUB), New town Sonargaon, Narayanganj. Bangladesh.

Md. Mustofa Nabi
Faculty of Unani and Ayurvedic Medicine, Hamdard University Bangladesh (HUB), New town Sonargaon, Narayanganj. Bangladesh.

Abdul Awal
Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An experiment was performed at the Department of Pharmacology in collaboration with the animal house of the Microbiology Department, Bangladesh Agricultural University, Mymensingh during the period from Jan 2005 to May 2005. The aim of this study, was to assess the comparative efficacy of Turmeric (Halud), Garlic (Rashun) and Aloe vera (Ghrita kumari) with Antibiotic Nebanol (Neomycin Sulphate and Bacitracin Zinc) on artificially induced wound in 2.5 cm length and 0.5 cm depth were made on the two thigh muscles an experimental model. Twenty five guinea pigs of both sexes age ranged between 6 to 8 months were divided into five groups (each group containing five animals). The first, second and third groups received herbal medicine e.g.; Turmeric paste, Garlic paste, Aloe gel and fourth group received Antibiotic Nebanol powder (Neomycin Sulphate and Bacitracin Zinc) respectively. The fifth/control groups were observed without medicine. Turmeric, Garlic, Aloe & Nebanol treatment reduced the wound within 12, 15, 17 & 14 days respectively. On the other hand, wound in control group was automatically reduced within 21 days. The present study showed that Turmeric was best effective among all and was found better than antibiotic Nebanol (Neomycin Sulphate and Bacitracin Zinc). It could thus be concluded that among 3 indigenous preparations, Turmeric may be used against skin wound because it is less expensive, easily available and have less side effect.

Keywords: Turmeric, Garlic, Aloe, Nebanol, Guinea pigs, Wound.

Location: Department of Pharmacology and Microbiology, Bangladesh Agricultural University, Mymensingh

Start Date: 00-01-2005

End Date: 00-05-2005

Program Area: Development of Host and Medicinal Plants

Commodity: Aloe Vera, Garlic, Turmeric

Objectives:

To find out the potential of Curcuma longa, Alium sativum, Aloe barbadensis in wound healing of Guinea Pigs
To determine the most suitable wound models through comparision

Methodology:

Total 25 number of Guinea Pigs of both sex,having weight of 450-600gm, age: 6-8 month were used for the study. The leaves of the Ghrita kumari (Alo vera) were collected from the Botanical garden of Bangladesh Agricultural University, Mymensingh and the raw Halud (Turmeric) and Rashun (Garlic) were purchased from the local market. The raw Halud and Rashun were cleansed with fresh water and air-dried. Then the raw Halud and Rashun were then mixed with distilled water and grinded them as 60% solution. The pastes were then preserved in the separate plastic container. The gel of the Ghrita kumari leaves were isolated from the leaves and kept in separate plastic container. Nebanol^® Powder (Neomycin Sulphate and Bacitracin Zinc): (Square Pharmaceuticals, Bangladesh) was purchased from local market. Before surgery, all the animals were subjected to a thorough clinical examination to ensure that they were in good physical condition and apparently free from infections or parasitic diseases. The operation sites both thighs were clipped, washed with soap and water, shaved and prepared with Iosan (Ciba Geigy, Switzerland Ltd). The sites were desensitized by local infiltration with 2% Jasocaine (Lignocaine hydrochloride, Jayson, Bangladesh Limited). Two incised wounds of 2.5 cm length and 0.5 cm depth were then made on either side of horizontal column following standard surgical procedure. The wounds produced in all experimental groups were kept unsutured.After making wounds, the guinea pigs were divided into 5 equal groups. Each comprising five animals and marked as group – I, II, III, IV and group V. Animals were maintained carefully so as to avoid interference with the formation of granulation tissue.

Group- I: Paste of Turmeric was applied to the 10 wounds made in 5 animals for twelve days. The paste was used twice daily after washing the wounds by distilled water with the help of sterile cotton.
Group- II: Paste of Garlic was applied to the 10 wounds made in 5 animals for fifteen days.
Group- III: The gel of Aloe leaves was applied to the 10 wounds made in 5 animals for seventeen days.
Group- IV: Antibiotic (Nebanol powder) was applied to the 10 wounds made in 5 animals for fourteen days.
Group- V: No medicine was applied to the 10 wounds made in 5 animals.

A drop of blood was collected from the ear vain of each experimental animal before wounding and on 3^rd, 10^th, and 14^th days of wounding. The blood was analyzed for Total Erythrocyte Count (TEC), Total Leukocyte Count (TLC) Haemoglobin (Hb) & Differential Leukocyte Counts (DLC). 7 The data were analyzed statistically between treatment and control values by using SPSS.

Source of Information: International Journal of Pharmaceutical Sciences and Research, 2015; Vol. 6(5): 2081-2089.

Article Link (Online Journal): http://ijpsr.com/?action=download_pdf&postid=20125

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Phytomedicine could be used for the treatment of diseases. Turmeric, Garlic, Aloe vera paste can industrially be manufactured for effective but inexpensive treatment of wounds.

Knowledge Management: Journal