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Screening of Genotypes/mutants/advance Lines/varieties of Rice for High Efficiency of N-utilization

R. K. Dutta
BINA, Mymensingh

Dr. Md. Badruddin
Crop Physiology Division, BINA, Mymensingh

Dr. Md. Tariqul Islam
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted with 33 rice genotypes at BINA, Mymensingh during July to December 1991 to identify rice genotypes for their status of nitrate reduction capacity among the existing genotypes. In rice, nitrate reduction capacity as expressed by nitrate reductase activity was high and the values were among 33 genotypes between 1.8 to 17.7 units with an average of 7.17 units and a variation of ± 4.1 units. Twenty three genotypes showed NR activity more than 4 units. The results suggested that most of the elite genotypes which were selected on the basis of better agronomic characters had also higher NR activity.

Keywords: Nitrate reductase, Nitrogen, Rice

Location: BINA, Mymensingh

Start Date: 00-07-1991

End Date: 00-12-1991

Program Area: Variety and Species

Commodity: Rice

Objectives:

To identify rice genotypes for their status of nitrate reduction capacity among the existing genotypes

Methodology:

The experiment was conducted with 33 rice genotypes at BINA, Mymensingh during July to December 1991. All the rice genotypes were collected from Plant Breeding Division, BINA, Mymensingh. The plants were grown in hydroponic culture under the glass house condition. The plants were regularly supplied with 0.25 strength long Ashton (Hewitt, 1966) nutrient solution without N in the medium up to the date at which nitrate was provided @ 5.0 mM 48 or 72 hours before the analysis of Nitrate reductase (NR). For the analysis of NR, 100 mg of selected leaf samples taken in a scintillation vial with 5.0 ml of freshly prepared assay solution containing 0.1M phosphate buffer of pH 7.5, 1.5% N-propanol, 0.05% Neutronix and 1.5% Potassium nitrate were vacuum infiltrated in a desiccator and dark incubated for 1 hour at 27^oC with moderate shaking. After 1 hour, 1.0 ml of extract was added to 2.0 ml of color reagent which contained 1% sulphanilic acid and 0.02% NEDD. Blank was prepared with 1.0 ml of assay solution with 2.00 ml of color reagents.

Source of Information: BINA Annual Report, 1991-1992: pages 99-105, Mymensingh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Selection of rice genotypes on the basis of NR activity at early growth stage might lead to the selection of rice genotypes of better performance as well as maximal soil nitrate utilization capacity.

Knowledge Management: Report/Proceedings