M. J. Islam
Assistant Professor
Department of Pharmacy, University of Development Alternative, Dhanmondi, Dhaka
S. Barua,
Department of Microbiology, University of Chittagong, Bangladesh
S. Das,
Department of Microbiology, University of Chittagong, Bangladesh
M. S. Khan And
Department of Microbiology, University of Chittagong, Bangladesh
A. Ahmed
Department of Pharmacy, University of Development Alternative, Dhaka, Bangladesh
Folk medicinal plants, Antibacterial activity, Crude ethanolic extract, Blumea lacera
Laboratory of Department of Microbiology, University of Chittagong
Resource Development and Management
Medicinal Plants
Plant materials: The Plants Callicarapa arborea,Lannea coromandelica,Ficus recemosa Linn,Streblus asperLour, Lawsonia inermis, Holarrhena antidysenterica, Mentha arvensis, Enhydra fluctuans, Blumea lacera, Glinus oppositifolius, Chenopodium album, Hemidesmus indicus, Coccinea cordifolia Linn, Cuscuta reflexa Roxb, Capparis zeylanica Linn and Kalanchoe pinnata were collected from the Sitakundu and Patiya, chittagong, Bangladesh in February, 2008 which were used for the treatment of infectious diseases by tribal peoples of Chittagong, Bangladesh. The plants were identified at the Bangladesh National Herbarium. The plant materials were oven-dried at 40ºC and then ground into coarse powder. Extraction: Briefly, The coarse powder of the Callicarapa arborea (20 g), Lannea coromandelica(20 g), Ficus recemosa Linn(30 g), Streblus asper Lou(35 g)r, Lawsonia inermis(41.0 g), Holarrhena antidysenterica(37.5g), Mentha arvensis(19.0 g), Enhydra fluctuans(25.0 g), Blumea lacera(31.0 g), Glinus oppositifolius(37.0g), Chenopodium album(33.0 g), Hemidesmus indicus(34.0 g), Coccinea cordifolia Linn(31.0 g), Cuscuta reflexa Roxb(34.0 g), Capparis zeylanica Linn(38.0 g) and Kalanchoe pinnata(42.0 g) were extracted with ethanol for a week at room temperature. The extracts were then filtered off through Whatman filter paper number-1 and the solvent was removed under vacuum at 30ºC until dry mass were obtained by Buchi rotavapor. Antibacterial Activity Test: Microorganisms: The bacteria used included: Shigella dysenteriae, Salmonella typhi, Salmonella paratyphi, Bacillus cerus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholerae, Bacillus megaterium. Bacterial cultures were maintained on Nutrient agar media. All cultures were sub-cultured monthly and subsequently stored at 4°C. Screening for Antimicrobial Activities: Disc diffusion method (Bauer et al., 1966) was used to test the antimicrobial activity of the extractives against ten bacteria. Dried and sterilized filter paper discs (6 mm diameter) were then impregnated with known amount of the test substances dissolved in ethanol (40 μg/ml) using micro pipette and the residual solvents were completely evaporated. Discs containing the test material (20 μg/disc) were placed on nutrient agar medium uniformly seeded with the test microorganisms. Standard disc of kanamycin (30 μg/disc) and blank discs (impregnated with solvents followed by evaporation) were used as positive and negative control, respectively. These plates were then kept at low temperature (4oC) for 24 hours to allow maximum diffusion of test samples. The plates were then incubated at 37oC for 24 hours to allow maximum growth of the organisms. The test materials having antimicrobial activity inhibited the growth of the microorganisms and a clear, distinct zone of inhibition was visualized surrounding the disc. The antimicrobial activity of the test agents was determined by measuring the diameter of zone of inhibition in millimeter. The experiment was carried out in triplicate and the average zone of inhibition was calculated.
Journal of Soil and Nature. 2 (3):26-28, 2008
Journal