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Cardinal Investigation on Phenolic and Antioxidant Content Analysis of Mixed Fruit Juices Marketed in Bangladesh

Md. D. Sohel
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.

Faisal Asif
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.

Tania Sultana
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.

Habiba Mohammadi
Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.

Md. H. U. Sumon
Department of Pharmacy, University of Development Alternative, Dhaka, Bangladesh.

Md. A. Rahman
Department of Pharmacy, East West University, Dhaka, Bangladesh .

Abstract/ Executive Summary:

Fresh fruits are convenient source of natural phenols and antioxidants which are healthy for human. At present many marketed fruit juices claims to be salubrious and full of phenols and antioxidants. In this study enlighten on investigation of phenols and antioxidant. Five mixed fruits juices were collected from Bangladeshi market for investigation. For the phenolic contents determination modified Folin–Ciocalteu and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) modified method for antioxidant analysis was applied. The value of phenolic content was S1 (43.88±0.51), S2 (54.29±0.55), S3 (79.97±1.15), S4 (69.97±1.02) a nd S5 (57.70±0.78) respectively. On basis of the IC 50 value was S1 (81.59±1.20), S2 (49.91±0.85), S3 (28.75±0.76), S4 (24.44±0.67) and S5 (61.075±0.95) respectively. This study signifies the determination of phenolic contents and antioxidants presence in mixed fruit juices with substantial activeness.

Keywords: Antioxidant, Mixed fruit juices, Phenolic content, Free radical scavenging activity

Location: Dhaka, Bangladesh

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Fresh and processed food

Objectives:

To examine phenolic contents and antioxidant of marketed fruit juices.

Methodology:

All analytical absorbance measurements were performed by a double beam Shimadzu UV-visible spectrometer (UV mini-1700, Shimadzu Corporation, Kyoto, Japan) with 1 cm quartz cell. Five samples of mixed fruit juices (freshly prepared not exceeding two weeks after manufacturing) were collected from market places of Dhaka, Bangladesh area . All samples were stored at 4⁰C throughout the study. The samples were checked for their license number, manufacturing batch number, manufacturing date and expiry dates. They were randomly numbered such as S1, S2, S3, S4 and S5 etc. The marketed fruit juices were filtered through 4-fold muslin cloth for existence of pulp in fruit juice contents. All analytical chemicals Folin–Ciocalteu’s phenol reagent (Sigma-Aldrich, Germany), gallic acid (Sigma–Aldrich Germany)1, 1-diphenyl-2-picrylhydrazyl (DPPH) (Merck, Darmstadt, Germany), Ascorbic acid (ASA) (Merck, Darmstadt, Germany), tert-butyl-1-hydroxytoluene (BHT) (Merck, Darmstadt, Germany) and other reagents were provided from Pharmaceutical Analysis Lab. State University of Bangladesh The modified Folin–Ciocalteu procedures (Marjorie, 2011). Gallic acid ranging from 500 µg/ml to 0 µg/ml prepared in distilled water. The modified Folin–Ciocalteu procedure consisted of transferring 50 µl standard or sample into a 4–5 ml test tube, followed by additions of 430 µl distilled water and 20 µl Folin–Ciocalteu reagent. After mixing the fruit juice samples, 50 µl 20% Na₂CO₃ and 450 µl distilled water were added. The sample mixtures were allowed to stand for 60 minutes at room temperature and were mixed after every addition of reagents. Aliquots of 200 µl were transferred to clear microtitre wells in duplicate and the absorbance was measured at 725 nm. The phenolic content of samples was measured against the gallic acid (GA) calibration standard (0–500 µg / ml) and the results were expressed as gallic acid equivalents (GAE). At first ascorbic acid (ASA) and tert-butyl-1-hydroxytoluene (BHT) as positive control was dissolve in methanol with serial dilution ranging from 500.0 to 0.977 µg /ml. The fruit juices sample (200 µl) assorted at different concentration (500 µg/ml to 0.977 µg/ml) were mixed with 3.0 ml of a DPPH methanol solution (20 µg/ml). After 30 min of reaction at room temperature in dark place the absorbance was measured at 517 nm against methanol as blank by UV spectrophotometer.

Source of Information: Der Chemica Sinica, 2014, 5(3):30-33, ISSN: 0976-8505

Article Link (Online Journal): www.pelagiaresearchlibrary.com

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

On the basis of study some mixed fruit juices contains reasonable amount of phenols and antioxidant. Due to commercial fruit juices using synthetic phenols and antioxidant there is pertaining issue of the activity on human body. Also high amount of synthetic phenols and antioxidant are vulnerable for human health. Further analysis and chemical studies is needed for the proper justification of mixed fruit juices.

Knowledge Management: Journal